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Anti-POU5F2 Antibody (aa71-120) LS-C109602

Catalog	Size	Price
LS-C109602-50	50 µg	Unavailable

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Anti-POU5F2 Antibody (APC) LS-C513832

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Anti-POU5F2 Antibody (Biotin) LS-C522046

Antibody:	Mouse Monoclonal (IgG1,k) to Human POU5F2
Reactivity:	Human
Application:	Western blot, ELISA
Format:	Biotin Conjugated

Antibody:	Rabbit Polyclonal to Human POU5F2
Reactivity:	Human
Application:	Western blot (applications tested for the base form of this product only)
Format:	Unconjugated

Details

Target:	Human POU5F2
Host:	Rabbit
Reactivity:	Human (tested or 100% immunogen sequence identity)
Clonality:	Polyclonal
Conjugations:	Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

Western blot (0.2 - 1 µg/ml)
(applications tested for the base form of this product only)

Specificity and Use

Immunogen:

POU5F2 antibody was raised against synthetic peptide located between aa71-120 of human POU5F2 (Q8N7G0, NP_694948). Percent identity by BLAST analysis: Human (100%).

Blocking Peptide:

LS-E11031 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C109602. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

aa71-120

Specificity:

Human POU5F2

Usage:

Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Presentation:	Lyophilized from PBS with 2% sucrose
Reconstitution:	Distilled water
Storage:	Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Restrictions:	For research use only.

About POU5F2

Uniprot: Q8N7G0 NCBI: Q8N7G0

Synonyms: POU5F2 Antibody, SPRM-1 Antibody, SPRM1 Antibody

Description: Transcription factor that binds preferentially to the octamer motif (5'-ATGTTAAT-3'). May exert a regulatory function in meiotic events that are required for terminal differentiation of male germ cell.

(More About POU5F2)

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Western blot

POU5F2 antibody LS-C109602 Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From:
Date Requested: 8/18/2017

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