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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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POU5F2 Antibody (aa71‑120) LS‑C109602
POU5F2 antibody LS-C109602 is an unconjugated rabbit polyclonal antibody to human POU5F2. Validated for WB.
Catalog
Size
Price
LS-C109602-50
50 µg
Unavailable

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Product Description

POU5F2 antibody LS-C109602 is an unconjugated rabbit polyclonal antibody to human POU5F2. Validated for WB.
About POU5F2
Transcription factor that binds preferentially to the octamer motif (5'-ATGTTAAT-3'). May exert a regulatory function in meiotic events that are required for terminal differentiation of male germ cell. Q8N7G0 Q8N7G0

POU5F2 Antibody, SPRM-1 Antibody, SPRM1 Antibody


Specifications

Target
Human POU5F2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
POU5F2 antibody was raised against synthetic peptide located between aa71-120 of human POU5F2 (Q8N7G0, NP_694948). Percent identity by BLAST analysis: Human (100%).
Blocking Peptide
LS-E11031 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C109602. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa71-120
Specificity
Human POU5F2
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

POU5F2 antibody LS-C109602 Western blot of THP-1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
POU5F2 antibody LS-C109602 Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

POU5F2 antibody LS-C109602 Western blot of THP-1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
POU5F2 antibody LS-C109602 Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

POU5F2 antibody LS-C109602 Western blot of THP-1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
POU5F2 antibody LS-C109602 Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

POU5F2 antibody LS-C109602 Western blot of THP-1 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
POU5F2 antibody LS-C109602 Western blot of THP-1 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/24/2018

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