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Anti-POU5F1 / OCT4 Antibody (Internal) LS-C60139

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular POU5F1 / OCT4 Antibodies

Anti-POU5F1 / OCT4 Antibody (Internal, Biotin) LS-C140990
Rabbit Polyclonal to Human POU5F1 / OCT4
Human
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Biotin Conjugated
Immunohistochemistry Image
Anti-POU5F1 / OCT4 Antibody (clone MRQ-10) LS-C202890
Mouse Monoclonal [clone MRQ-10] (IgG1) to Human POU5F1 / OCT4
Human
IHC - Paraffin, IHC - Frozen
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human POU5F1 / OCT4
Human, Monkey, Horse
Western blot, ELISA
Unconjugated

Details

Human POU5F1 / OCT4
Rabbit
Human, Monkey, Horse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:1000 - 1:1500)
  • ELISA (1:350000)

Specificity and Use

POU5F1 / OCT4 antibody was raised against synthetic peptide corresponding to an internal region of human Oct-4 protein.
Internal
human and mouse Oct-4 protein. A BLAST analysis was used to suggest additional cross-reactivity with Oct-4 from chimpanzee, macaque and horse based on a 100% homology with the immunizing sequence; and mink, dog, bovine and pig based on a 93% homology. Cross-reactivity with Oct-4 from other sources has not been determined
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~39 kD in size corresponding to Oct-4 by western blotting in the appropriate cell lysate or extract. This antibody is capable of detecting endogenous Oct-4.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: -20°C
For research use only.

About POU5F1 / OCT4

Q01860 NM_002701 NP_002692.2

POU5F1 Antibody, Oct-4 Antibody, OCT3 Antibody, OCT4 Antibody, OTF4 Antibody, POU class 5 homeobox 1 Antibody, Octamer-binding protein 3 Antibody, Octamer-binding protein 4 Antibody, OTF-3 Antibody, Oct-3 Antibody, OTF3 Antibody

POU5F1 / OCT4 is a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms.

Western blot

Western blot
Anti-Oct4 Antibody - Western Blot. Western blot of affinity purified anti-Oct4 antibody shows detection of endogenous Oct4 in mouse embryonic fibroblast (MEF) cell lysate (lane 1) and HeLa nuclear extract (HN) (lane 2). The band at ~39 kD (arrow head) corresponds to Oct4. After transfer, the membrane was blocked with 5% BSA. Primary antibody was used at a 1:1000 dilution in PBS containing 5% BSA. The specificity of the antibody was confirmed by peptide competition which blocks reaction of the antibody with Oct4 (lanes 3 and 4, respectively).
 

Requested From: United States
Date Requested: 12/3/2016

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