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| Catalog Number | Size | Price |
|---|---|---|
| LS-C60139-100 | 100 µg (1 mg/ml) | $567 |
Polyclonal Rabbit anti‑Human POU5F1 / OCT4 Antibody (IF, WB) LS‑C60139
Polyclonal Rabbit anti‑Human POU5F1 / OCT4 Antibody (IF, WB) LS‑C60139
Antibody:
POU5F1 / OCT4 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, IP, ELISA
Reactivity:
Human, Monkey, Mouse, Horse
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
POU5F1 / OCT4 Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, IP, ELISA
Reactivity:
Human, Monkey, Mouse, Horse
Format:
Unconjugated, Unmodified
Specifications
Description
OCT4 antibody LS-C60139 is an unconjugated rabbit polyclonal antibody to OCT4 (POU5F1) from human. It is reactive with human, mouse, horse and other species. Validated for ELISA, IF, IP and WB.
Target
Human POU5F1 / OCT4
Synonyms
POU5F1 | Oct-4 | OCT3 | OCT4 | OTF4 | POU class 5 homeobox 1 | Octamer-binding protein 3 | Octamer-binding protein 4 | OTF-3 | Oct-3 | OTF3 | OCT3/4
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Horse
(tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Rat, Bovine, Dog, Mink, Pig, Macaque (at least 90% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Oct-4 protein.
Specificity
This antibody reacts with human and mouse Oct-4 protein. Cross-reactivity with Oct-4 from other species has not been tested. A BLAST analysis was used to suggest additional cross-reactivity with Oct-4 from chimpanzee, macaque and horse based on a 100% homology with the immunizing sequence; and mink, dog, bovine and pig based on a 93% homology.
Applications
- Immunofluorescence
- Western blot (1:1000 - 1:1500)
- Immunoprecipitation
- ELISA (1:350000)
Usage
This affinity purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~39 kD in size corresponding to Oct-4 by western blotting in the appropriate cell lysate or extract. This antibody is capable of detecting endogenous Oct-4.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About POU5F1 / OCT4
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat, Primate
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human, Mouse, Porcine
Applications:
IHC, IHC - Paraffin, IHC - Frozen, ICC, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen
Species:
Human, Rodent
Applications:
IHC, ICC, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/23/2024
Date Requested: 4/23/2024
