Human POU5F1 / OCT4
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
- IHC (1:200 - 1:1000)
- ICC (1:200 - 1:1000)
- Western blot (1:200 - 1:1000)
- (applications tested for the base form of this product only)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
POU5F1 / OCT4 antibody was raised against synthetic peptide (aa 282-297) of Oct4.
Recognizes Oct4 (POU5F1). MW: 38,.6kD. Species cross-reactivity: Human and rodent.
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Western Blot: 1:200-1:1000 using ECL. Immunohistochemistry: 1:200-1:1000. Immunocytochemistry: 1:200-1:1000.Immunohistochemistry protocol:1. Dissect tissues and freeze on dry ice.2. Cut on a cryostat 10um slices.3. Dry slides at RT for 30-90 min4. Fix with cold Acetone: Methanol (50:50) 2 min. Either process further or store at -20ºC. Alternative is fixation with 4% PFA and treatment with 0.05% trypsin solution (incubation 10 min. at 37ºC, followed by 3 washes with PBS)5. Let air dry.6. PBS 5 min7. Incubate in 50mM ammonium chloride 30 min.8. PBS 5 min9. Blocking serum 30-45 min10. Primary antibody (LS-C9379) 90 min11. Wash 3X for 5 min12. Secondary fluorescent conjugated antibody 30 min13. Wash 3X for 5 min.14. Mount.
PBS, pH 7.4, No preservatives added
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.