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Anti-POU5F1 / OCT4 Antibody (aa282-297) LS-C9379

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Wt. Vol. Conc. Price
100 µg - 1 mg/ml $560
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Rabbit Polyclonal (IgG) to Human POU5F1 / OCT4
Human, Rodent
IHC, ICC, Western blot
Unconjugated

Details

Human POU5F1 / OCT4
Rabbit
Human, Rodent (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified

Applications

IHC (1:200 - 1:1000), ICC (1:200 - 1:1000), Western blot (1:200 - 1:1000)

Specificity and Use

POU5F1 / OCT4 antibody was raised against synthetic peptide (aa 282-297) of Oct4.
aa282-297
Recognizes Oct4 (POU5F1). MW: 38,.6kD. Species cross-reactivity: Human and rodent.
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Western Blot: 1:200-1:1000 using ECL. Immunohistochemistry: 1:200-1:1000. Immunocytochemistry: 1:200-1:1000.Immunohistochemistry protocol:1. Dissect tissues and freeze on dry ice.2. Cut on a cryostat 10um slices.3. Dry slides at RT for 30-90 min4. Fix with cold Acetone: Methanol (50:50) 2 min. Either process further or store at -20ºC. Alternative is fixation with 4% PFA and treatment with 0.05% trypsin solution (incubation 10 min. at 37ºC, followed by 3 washes with PBS)5. Let air dry.6. PBS 5 min7. Incubate in 50mM ammonium chloride 30 min.8. PBS 5 min9. Blocking serum 30-45 min10. Primary antibody (LS-C9379) 90 min11. Wash 3X for 5 min12. Secondary fluorescent conjugated antibody 30 min13. Wash 3X for 5 min.14. Mount.

Packaging

PBS, pH 7.4. No preservative added.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About POU5F1 / OCT4

Q01860 NM_002701 NP_002692.2

POU5F1 Antibody, Oct-4 Antibody, OCT3 Antibody, OCT4 Antibody, OTF4 Antibody, POU class 5 homeobox 1 Antibody, Octamer-binding protein 3 Antibody, Octamer-binding protein 4 Antibody, OTF-3 Antibody, Oct-3 Antibody, OTF3 Antibody

POU5F1 / OCT4 is a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms.

Requested From: United States
Date Requested: 9/30/2016

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