OCT4 antibody LS-C9379 is an unconjugated rabbit polyclonal antibody to OCT4 (POU5F1) from human and rodent. Validated for ICC, IHC and WB.
Human POU5F1 / OCT4
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
- IHC (1:200 - 1:1000)
- ICC (1:200 - 1:1000)
- Western blot (1:200 - 1:1000)
- (applications tested for the base form of this product only)
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POU5F1 / OCT4 antibody was raised against synthetic peptide (aa 282-297) of Oct4.
Recognizes Oct4 (POU5F1). MW: 38,.6kD. Species cross-reactivity: Human and rodent.
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Western Blot: 1:200-1:1000 using ECL. Immunohistochemistry: 1:200-1:1000. Immunocytochemistry: 1:200-1:1000.Immunohistochemistry protocol:1. Dissect tissues and freeze on dry ice.2. Cut on a cryostat 10um slices.3. Dry slides at RT for 30-90 min4. Fix with cold Acetone: Methanol (50:50) 2 min. Either process further or store at -20ºC. Alternative is fixation with 4% PFA and treatment with 0.05% trypsin solution (incubation 10 min. at 37ºC, followed by 3 washes with PBS)5. Let air dry.6. PBS 5 min7. Incubate in 50mM ammonium chloride 30 min.8. PBS 5 min9. Blocking serum 30-45 min10. Primary antibody (LS-C9379) 90 min11. Wash 3X for 5 min12. Secondary fluorescent conjugated antibody 30 min13. Wash 3X for 5 min.14. Mount.
PBS, pH 7.4, No preservatives added
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.