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Catalog Number Size Price
LS-C97712-400 400 µl $393 
POU5F1 / OCT4 Antibody - Fluorescent confocal image of SY5Y cells stained OCT4 (E125) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated OCT4 (E125) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min). OCT4 immunoreactivity is localized mainly to the nuclei and also to the cytoplasm.
POU5F1 / OCT4 Antibody - Western blot of OCT3/4 Antibody (E125) in HL60 cell line lysates (35 ug/lane). OCT3/4 (arrow) was detected using the purified antibody.
POU5F1 / OCT4 Antibody - Fluorescent confocal image of SY5Y cells stained OCT4 (E125) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated OCT4 (E125) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 mu M, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 3 min). OCT4 immunoreactivity is localized mainly to the nuclei and also to the cytoplasm.
POU5F1 / OCT4 Antibody - Western blot of OCT3/4 Antibody (E125) in HL60 cell line lysates (35 ug/lane). OCT3/4 (arrow) was detected using the purified antibody.
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Polyclonal Rabbit anti‑Human POU5F1 / OCT4 Antibody (aa110‑141, IHC, IF, WB) LS‑C97712

Polyclonal Rabbit anti‑Human POU5F1 / OCT4 Antibody (aa110‑141, IHC, IF, WB) LS‑C97712

Antibody:
POU5F1 / OCT4 Rabbit anti-Human Polyclonal (aa110-141) Antibody
Application:
IHC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C97712-400
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
POU5F1 / OCT4 Rabbit anti-Human Polyclonal (aa110-141) Antibody
Application:
IHC, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
OCT4 antibody LS-C97712 is an unconjugated rabbit polyclonal antibody to human OCT4 (POU5F1) (aa110-141). Validated for IF, IHC and WB.
Target
Human POU5F1 / OCT4
Synonyms
POU5F1 | Oct-4 | OCT3 | OCT4 | OTF4 | POU class 5 homeobox 1 | Octamer-binding protein 3 | Octamer-binding protein 4 | OTF-3 | Oct-3 | OTF3 | OCT3/4
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa110-141
Specificity
This OCT4 (OCT3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-141 amino acids from human OCT4 (OCT3).
Applications
  • IHC
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About POU5F1 / OCT4
Q01860 NM_002701 NP_002692.2

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 4/18/2024