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| Catalog Number | Size | Price |
|---|---|---|
| LS-B85-0.1 | 0.1 ml (1 mg/ml) | $460 |
IHC‑plus™ Polyclonal Rabbit anti‑Human POU5F1 / OCT4 Antibody (aa100‑200, IHC, IF, WB) LS‑B85
IHC‑plus™ Polyclonal Rabbit anti‑Human POU5F1 / OCT4 Antibody (aa100‑200, IHC, IF, WB) LS‑B85
Note: This antibody replaces LS-C2740
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Specifications
Description
OCT4 antibody LS-B85 is an unconjugated rabbit polyclonal antibody to OCT4 (POU5F1) (aa100-200) from human. It is reactive with human, mouse and porcine. Validated for Flow, ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human POU5F1 / OCT4
Synonyms
POU5F1 | Oct-4 | OCT3 | OCT4 | OTF4 | POU class 5 homeobox 1 | Octamer-binding protein 3 | Octamer-binding protein 4 | OTF-3 | Oct-3 | OTF3 | OCT3/4
Host
Rabbit
Reactivity
Human, Mouse, Porcine
(tested or 100% immunogen sequence identity)
Predicted
Bovine (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide made to an internal portion of the human OCT4 protein sequence (between residues 100-200). Percent identity by BLAST analysis: Bovine, Cat, Porcine (93%).
Epitope
aa100-200
Specificity
OCT4 - Embryonic Stem Cell Marker.
Applications
- IHC
- IHC - Paraffin (2.5 µg/ml)
- IHC - Frozen
- ICC (1:50 - 1:200)
- Immunofluorescence (1:50 - 1:200)
- Western blot (2 µg/ml)
- Flow Cytometry (1:50 - 1:200)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B85 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B85 was determined to be 2.5 ug/ml.
Presentation
Tris-Glycine, 150 mM NaCl, 0.05% Sodium Azide
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About POU5F1 / OCT4
Validation
Anti-POU5F1 / OCT4 antibody IHC of human breast carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-POU5F1 / OCT4 antibody IHC of human breast carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
IHC-plus™ POU5F1 / OCT4 Antibody (aa100-200) for IHC, ICC, IF/Immunofluorescence, WB/Western, Flow LS-B85 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Stabilization of HIF-1a and HIF-2a, up-regulation of MYCC and accumulation of stabilized p53 constitute hallmarks of CNS-PNET animal model. Malchenko S, Sredni ST, Bi Y, Margaryan NV, Boyineni J, Mohanam I, Tomita T, Davuluri RV, Soares MB. PloS one. 2017 12:e0173106. (IHC, WB; Mouse)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse, Rat, Primate
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen
Species:
Human, Rodent
Applications:
IHC, ICC, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
