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POU3F3 / BRN1 Antibody (aa35‑84) LS‑C109736
BRN1 antibody LS-C109736 is an unconjugated rabbit polyclonal antibody to BRN1 (POU3F3) from human, mouse and rat. Validated for WB.
Catalog
Size
Price
LS-C109736-50
50 µg
Unavailable

Popular POU3F3 / BRN1 Products

POU3F3 antibody (1 ug/ml) staining of Mouse Spinal Cord lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Species: Human, Mouse, Rat, Chicken, Xenopus
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
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Applications: Western blot, ELISA
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Applications: Western blot, ELISA
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Species: Human
Applications: Western blot, ELISA

Product Description

BRN1 antibody LS-C109736 is an unconjugated rabbit polyclonal antibody to BRN1 (POU3F3) from human, mouse and rat. Validated for WB.
About POU3F3 / BRN1
Transcription factor that acts synergistically with SOX11 and SOX4. Plays a role in neuronal development. Is implicated in an enhancer activity at the embryonic met-mesencephalic junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-3'). P20264 NM_006236 NP_006227.1

POU3F3 Antibody, Brain-1 Antibody, BRN1 Antibody, OTF8 Antibody, OTF-8 Antibody, POU class 3 homeobox 3 Antibody, Brn-1 Antibody, Oct-8 Antibody, Octamer-binding protein 8 Antibody


Specifications

Target
Human POU3F3 / BRN1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
POU3F3 / BRN1 antibody was raised against synthetic peptide located between aa35-84 of human POU3F3 (P20264, NP_006227). Percent identity by BLAST analysis: Human, Mouse, Rat, Opossum (100%).
Blocking Peptide
LS-E11646 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C109736. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa35-84
Specificity
Human POU3F3
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

POU3F3 antibody LS-C109736 Western blot of OVCAR-3 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
POU3F3 antibody LS-C109736 Western blot of OVCAR-3 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

POU3F3 antibody LS-C109736 Western blot of OVCAR-3 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
POU3F3 antibody LS-C109736 Western blot of OVCAR-3 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

POU3F3 antibody LS-C109736 Western blot of OVCAR-3 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
POU3F3 antibody LS-C109736 Western blot of OVCAR-3 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

POU3F3 antibody LS-C109736 Western blot of OVCAR-3 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
POU3F3 antibody LS-C109736 Western blot of OVCAR-3 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/18/2018

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