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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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POU2F1 / OCT1 Antibody (aa364‑391) LS‑C163874
OCT1 antibody LS-C163874 is an unconjugated rabbit polyclonal antibody to human OCT1 (POU2F1). Validated for IHC and WB.
Catalog
Size
Price
LS-C163874-400
400 µl
Unavailable

Popular POU2F1 / OCT1 Products

Antibody
Species: Human
Applications: Western blot, Immunoprecipitation, Gel shift
Western blot of OCT1/OTF1 in Jurkat cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) NIH 3T3 cell lysate using LS-C148603 at 2 ug/ml, 2 ug/ml and 4 ug/ml respectively. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.
Species: Human, Mouse, Rat, Bovine, Horse
Applications: Western blot
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-POU2F1 mAb.
Species: Human
Applications: ICC, Immunofluorescence, Western blot, Dot Blot
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Bat, Guinea pig, Horse, Pig, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Western blot

Product Description

OCT1 antibody LS-C163874 is an unconjugated rabbit polyclonal antibody to human OCT1 (POU2F1). Validated for IHC and WB.
About POU2F1 / OCT1
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). P14859 NM_002697 NP_002688.3

POU2F1 Antibody, NF-A1 Antibody, OCT1 Antibody, Octamer-binding protein 1 Antibody, Oct-1B Antibody, Oct-1 Antibody, OTF-1 Antibody, OTF1 Antibody, POU class 2 homeobox 1 Antibody


Specifications

Target
Human POU2F1 / OCT1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Pig, Chicken, Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E1165 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C163874. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa364-391
Specificity
This POU2F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-391 amino acids from the Central region of human POU2F1.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

POU2F1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human rectum tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
POU2F1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human rectum tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

POU2F1 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The POU2F1 antibody detected the POU2F1 protein (arrow).
POU2F1 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The POU2F1 antibody detected the POU2F1 protein (arrow).

Immunohistochemistry

POU2F1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human rectum tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
POU2F1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human rectum tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

POU2F1 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The POU2F1 antibody detected the POU2F1 protein (arrow).
POU2F1 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The POU2F1 antibody detected the POU2F1 protein (arrow).

Immunohistochemistry

POU2F1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human rectum tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
POU2F1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human rectum tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

POU2F1 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The POU2F1 antibody detected the POU2F1 protein (arrow).
POU2F1 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The POU2F1 antibody detected the POU2F1 protein (arrow).

Immunohistochemistry

POU2F1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human rectum tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
POU2F1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human rectum tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

POU2F1 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The POU2F1 antibody detected the POU2F1 protein (arrow).
POU2F1 Antibody western blot of HL-60 cell line lysates (35 ug/lane). The POU2F1 antibody detected the POU2F1 protein (arrow).


Requested From: 
Date Requested: 6/19/2018

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