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Anti-POU2AF1 / BOB1 Antibody (aa241-254) IHC-plus™ LS-B8331

Note: This antibody replaces LS-C139798

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular POU2AF1 / BOB1 Antibodies

Anti-POU2AF1 / BOB1 Antibody (aa1-27, FITC) LS-C266740
Rabbit Polyclonal (IgG) to Human POU2AF1 / BOB1
Human
IHC, Western blot, Flow Cytometry, ELISA
FITC Conjugated
Anti-POU2AF1 / BOB1 Antibody (aa238-256) LS-C312663
Rabbit Polyclonal (IgG) to Mouse POU2AF1 / BOB1
Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human POU2AF1 / BOB1
Human, Monkey, Bat, Horse
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human POU2AF1 / BOB1
Goat
Human, Monkey, Bat, Horse (tested or 100% immunogen sequence identity)
Bovine, Dog (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (1 - 2 µg/ml)
  • ELISA (1:8000)

Specificity and Use

POU2AF1 / BOB1 antibody was raised against synthetic peptide C-DSDAYALNHTLSVE from the C-terminus of human POU2AF1 / BOB1 (NP_006226.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Marmoset, Tamarin, Bat, Horse (100%); Gibbon, Panda, Dog, Bovine (93%); Elephant, Rabbit (86%).
LS-E27354 - Lyophilized - 100 µg - $145.00
aa241-254
Human POU2AF1 / BOB1.
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: In transfected HEK293 transiently expressing full-length Human POU2AF1 (myc and DYKDDDDK tagged), a band of approx. 38kD was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About POU2AF1 / BOB1

Q16633 NM_006235 NP_006226.2

POU2AF1 Antibody, BOB-1 Antibody, OBF-1 Antibody, OCT-binding factor 1 Antibody, BOB1 Antibody, OBF1 Antibody, OCA-B Antibody, OCAB Antibody

Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.

Immunohistochemistry

Immunohistochemistry
Anti-POU2AF1 / BOB1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8331 dilution 3.75 ug/ml.
 

Requested From: United States
Date Requested: 12/3/2016

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