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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PORCN Antibody (aa224‑239) LS‑C204273
PORCN antibody LS-C204273 is an unconjugated rabbit polyclonal antibody to human PORCN. Validated for Flow, IF and WB.
Catalog
Size
Price
LS-C204273-1
1 ea
Unavailable

Popular PORCN Products

HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Applications: Western blot
PORCN antibody LS-C145383 Western blot of Mouse Thymus lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse, Human, Monkey, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Applications: Western blot
Immunohistochemistry of paraffin-embedded human kidney using antibody at dilution of 1:100.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Antibody
Species: Human, Mouse
Applications: IHC, Western blot, ELISA

Product Description

PORCN antibody LS-C204273 is an unconjugated rabbit polyclonal antibody to human PORCN. Validated for Flow, IF and WB.
About PORCN
PORCN belongs to the evolutionarily conserved porcupine (Porc) gene family. Genes of the porcupine family encode endoplasmic reticulum proteins with multiple transmembrane domains. Porcupine proteins are involved in the processing of Wnt (wingless and int homologue) proteins. Disruption of this gene is associated with focal dermal hypoplasia, and the encoded protein has been implicated in cancer. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. Q9H237 NM_022825 NP_073736.2

PORCN Antibody, PORC Antibody, Porcupine homolog (Drosophila) Antibody, PPN Antibody, DHOF Antibody, FODH Antibody, MG61 Antibody, Protein MG61 Antibody


Specifications

Target
Human PORCN
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:200)
  • Flow Cytometry (1:50 - 1:100)
Immunogen
PORCN antibody was raised against human PORCN amino acids 224-239.
Epitope
aa224-239
Specificity
Human PORCN / POR
Presentation
Lyophilized from TBS, pH 7.4.
Reconstitution
1 ea Reconstitute with 500 ul deionized water
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunofluorescence

Immunofluorescent staining of HeLa cells with PORCN antibody (10 ug/ml)
Immunofluorescent staining of HeLa cells with PORCN antibody (10 ug/ml)

Western blot

Western blot with PORCN antibody at 4 ug/ml. Lanes 1 and 3: MW markers, Lane 2: A549 cell lysate (20 ug), Lane 4: A549 cell lysate (20 ug) + PORCN peptide (20 ug/ml).
Western blot with PORCN antibody at 4 ug/ml. Lanes 1 and 3: MW markers, Lane 2: A549 cell lysate (20 ug), Lane 4: A549 cell lysate (20 ug) + PORCN peptide (20 ug/ml).

Flow Cytometry

Flow cytometry of HeLa cells with PORCN antibody. Yellow: 5 ug/ml, Green: 10 ug/ml, Blue: Normal rabbit IgG-FITC, Red: Goat anti-rabbit IgG FITC (1:200).
Flow cytometry of HeLa cells with PORCN antibody. Yellow: 5 ug/ml, Green: 10 ug/ml, Blue: Normal rabbit IgG-FITC, Red: Goat anti-rabbit IgG FITC (1:200).

Immunofluorescence

Immunofluorescent staining of HeLa cells with PORCN antibody (10 ug/ml)
Immunofluorescent staining of HeLa cells with PORCN antibody (10 ug/ml)

Western blot

Western blot with PORCN antibody at 4 ug/ml. Lanes 1 and 3: MW markers, Lane 2: A549 cell lysate (20 ug), Lane 4: A549 cell lysate (20 ug) + PORCN peptide (20 ug/ml).
Western blot with PORCN antibody at 4 ug/ml. Lanes 1 and 3: MW markers, Lane 2: A549 cell lysate (20 ug), Lane 4: A549 cell lysate (20 ug) + PORCN peptide (20 ug/ml).

Flow Cytometry

Flow cytometry of HeLa cells with PORCN antibody. Yellow: 5 ug/ml, Green: 10 ug/ml, Blue: Normal rabbit IgG-FITC, Red: Goat anti-rabbit IgG FITC (1:200).
Flow cytometry of HeLa cells with PORCN antibody. Yellow: 5 ug/ml, Green: 10 ug/ml, Blue: Normal rabbit IgG-FITC, Red: Goat anti-rabbit IgG FITC (1:200).

Immunofluorescence

Immunofluorescent staining of HeLa cells with PORCN antibody (10 ug/ml)
Immunofluorescent staining of HeLa cells with PORCN antibody (10 ug/ml)

Western blot

Western blot with PORCN antibody at 4 ug/ml. Lanes 1 and 3: MW markers, Lane 2: A549 cell lysate (20 ug), Lane 4: A549 cell lysate (20 ug) + PORCN peptide (20 ug/ml).
Western blot with PORCN antibody at 4 ug/ml. Lanes 1 and 3: MW markers, Lane 2: A549 cell lysate (20 ug), Lane 4: A549 cell lysate (20 ug) + PORCN peptide (20 ug/ml).

Flow Cytometry

Flow cytometry of HeLa cells with PORCN antibody. Yellow: 5 ug/ml, Green: 10 ug/ml, Blue: Normal rabbit IgG-FITC, Red: Goat anti-rabbit IgG FITC (1:200).
Flow cytometry of HeLa cells with PORCN antibody. Yellow: 5 ug/ml, Green: 10 ug/ml, Blue: Normal rabbit IgG-FITC, Red: Goat anti-rabbit IgG FITC (1:200).

Immunofluorescence

Immunofluorescent staining of HeLa cells with PORCN antibody (10 ug/ml)
Immunofluorescent staining of HeLa cells with PORCN antibody (10 ug/ml)

Western blot

Western blot with PORCN antibody at 4 ug/ml. Lanes 1 and 3: MW markers, Lane 2: A549 cell lysate (20 ug), Lane 4: A549 cell lysate (20 ug) + PORCN peptide (20 ug/ml).
Western blot with PORCN antibody at 4 ug/ml. Lanes 1 and 3: MW markers, Lane 2: A549 cell lysate (20 ug), Lane 4: A549 cell lysate (20 ug) + PORCN peptide (20 ug/ml).

Flow Cytometry

Flow cytometry of HeLa cells with PORCN antibody. Yellow: 5 ug/ml, Green: 10 ug/ml, Blue: Normal rabbit IgG-FITC, Red: Goat anti-rabbit IgG FITC (1:200).
Flow cytometry of HeLa cells with PORCN antibody. Yellow: 5 ug/ml, Green: 10 ug/ml, Blue: Normal rabbit IgG-FITC, Red: Goat anti-rabbit IgG FITC (1:200).


Requested From: 
Date Requested: 6/24/2018

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