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Anti-POLR3E / SIN Antibody LS-C155686

Catalog Size Price
LS-C155686-100 100 µl (1 mg/ml) Unavailable

Related Products

12 POLR3E / SIN Antibodies

Most Popular POLR3E / SIN Antibodies

Anti-POLR3E / SIN Antibody (aa191-240) LS-C119920
Rabbit Polyclonal (IgG) to Human POLR3E / SIN
Human, Mouse
IHC, Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Anti-POLR3E / SIN Antibody LS-C155686
Rabbit Polyclonal (IgG) to Human POLR3E / SIN
Human
ICC, Immunofluorescence, Western blot
Unconjugated
Immunofluorescence of paraformaldehyde-fixed A431, using POLR3E antibody at 1:500 dilution.
Anti-POLR3E / SIN Antibody (clone 2791C3a) LS-C342864
Mouse Monoclonal [clone 2791C3a] (IgG2b) to Human POLR3E / SIN
Human
ICC, Western blot, Dot Blot
Unconjugated
Western blot analysis of immunized recombinant protein, using anti-POLR3E monoclonal antibody.
Anti-POLR3E / SIN Antibody (Internal) LS-C368900
Rabbit Polyclonal to Human POLR3E / SIN
Human, Monkey
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemical analysis of POLR3E staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Anti-POLR3E / SIN Antibody (aa160-240) LS-C385585
Rabbit Polyclonal (IgG) to Human POLR3E / SIN
Human, Mouse
IHC, Immunofluorescence, Western blot, ELISA
Unconjugated
Western blot of POLR3E antibody

100% Guaranteed
Rabbit Polyclonal (IgG) to Human POLR3E / SIN
Human
ICC, Immunofluorescence, Western blot
Unconjugated

Details

Human POLR3E / SIN
Rabbit
Human (tested or 100% immunogen sequence identity)
Mouse, Rat (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • ICC (1:100 - 1:1000)
  • Immunofluorescence (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)

Specificity and Use

POLR3E / SIN antibody was raised against recombinant fragment corresponding to a region within amino acids 89 and 323 of POLR3E (SwissProt Q9NVU0). Percent identity by BLAST analysis: Human (100%); Mouse, Rat (96%); Zebrafish (82%).
Human POLR3E
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Packaging

PBS, pH 7, 1% BSA, 20% Glycerol, 0.01% Thimerosal
Aliquot and freeze at -20°C. Avoid freeze-thaw cycles.
For research use only.

About POLR3E / SIN

Q9NVU0 NM_018119 NP_060589.1

POLR3E Antibody, KIAA1452 Antibody, RNA polymerase III subunit C5 Antibody, RPC5 Antibody, SIN Antibody

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Essential for efficient transcription from both the type 2 VAI and type 3 U6 RNA polymerase III promoters. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses.

Immunofluorescence

Immunofluorescence of paraformaldehyde-fixed A431, using POLR3E antibody at 1:500 dilution.
Immunofluorescence of paraformaldehyde-fixed A431, using POLR3E antibody at 1:500 dilution.

Western blot

Sample (30 ug of whole cell lysate) A: Jurkat 7.5% SDS PAGE POLR3E antibody diluted at 1:1000
Sample (30 ug of whole cell lysate) A: Jurkat 7.5% SDS PAGE POLR3E antibody diluted at 1:1000

Requested From: 
Date Requested: 5/26/2018

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