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Anti-POLR3E / SIN Antibody LS-C155686

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular POLR3E / SIN Antibodies

Anti-POLR3E / SIN Antibody LS-C126144
Mouse Monoclonal (IgG2b) to Human POLR3E / SIN
Human
Western blot
Unconjugated
Anti-POLR3E / SIN Antibody (clone 2791C3a) LS-C184742
Mouse Monoclonal [clone 2791C3a] (IgG2b) to Human POLR3E / SIN
Human
ICC, Dot Blot
Unconjugated
Anti-POLR3E / SIN Antibody (clone 2791C3a) LS-C342864
Mouse Monoclonal [clone 2791C3a] (IgG2b) to Human POLR3E / SIN
Human
ICC, Western blot, Dot Blot
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human POLR3E / SIN
Human
ICC, Immunofluorescence, Western blot
Unconjugated

Details

Human POLR3E / SIN
Rabbit
Human (tested or 100% immunogen sequence identity)
Mouse, Rat (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • ICC (1:100 - 1:1000)
  • Immunofluorescence (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)

Specificity and Use

POLR3E / SIN antibody was raised against recombinant fragment corresponding to a region within amino acids 89 and 323 of POLR3E (SwissProt Q9NVU0). Percent identity by BLAST analysis: Human (100%); Mouse, Rat (96%); Zebrafish (82%).
Human POLR3E
Suggested starting dilutions are as follows: Western blot: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Packaging

PBS, pH 7, 1% BSA, 20% Glycerol, 0.01% Thimerosal
Aliquot and freeze at -20° C. Avoid freeze-thaw cycles.
For research use only.

About POLR3E / SIN

Q9NVU0 NM_018119 NP_060589.1

POLR3E Antibody, KIAA1452 Antibody, RNA polymerase III subunit C5 Antibody, RPC5 Antibody, SIN Antibody

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Essential for efficient transcription from both the type 2 VAI and type 3 U6 RNA polymerase III promoters. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses.

Immunofluorescence

Immunofluorescence
Immunofluorescence of paraformaldehyde-fixed A431, using POLR3E antibody at 1:500 dilution.

Western blot

Western blot
Sample (30 ug of whole cell lysate) A: Jurkat 7.5% SDS PAGE POLR3E antibody diluted at 1:1000

Requested From: United States
Date Requested: 12/5/2016

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