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POLR2F

polymerase (RNA) II (DNA directed) polypeptide F

DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II, and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2F/RPB6 is part of the clamp element and together with parts of RPB1 and RPB2 forms a pocket to which the RPB4-RPB7 subcomplex binds.

Gene Name: polymerase (RNA) II (DNA directed) polypeptide F
Synonyms: POLR2F, RPB6, RPB6 homolog, RPC15, POLRF, RPABC2, HRBP14.4, RPABC14.4, RPB14.4
Target Sequences: NM_021974 NP_068809.1 P61218

☰ Filters
Antibodies (15)
Proteins (6)
Over-Expression Lysate (2)
Primary (15)
Recombinant (4)
POLR2F (21)
Human (13)
Mouse (6)
Rat (5)
IHC (5)
WB (15)
ELISA (4)
ICC (1)
IF (6)
Peptide-ELISA (1)
rabbit (12)
mouse (3)
Ig (1)
IgG (8)
IgG1 (2)
IgG2a,k (1)
monoclonal mc (3)
polyclonal pc (12)
2G2 (1)
OTI2F2 (2)
Carrier-free (1)
Unconjugated (15)
N-Terminus (1)
No (21)
GST (1)
GST, N-terminus (1)
His (1)
Myc-DDK (Flag) (3)
Human (5)
293T Cells (1)
E. coli (1)
HEK 293 Cells (2)
Wheat Germ Extract (2)
Purified (1)
Select
POLR2F Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, WB
Unconjugated
100 µg/$331
POLR2F Antibody - Immunofluorescence of monoclonal antibody to POLR2F on HeLa cell . [antibody concentration 10 ug/ml]
Select
POLR2F Mouse anti-Human Monoclonal (2G2) Antibody
Human
ELISA, IF, WB
Unconjugated
100 µg/$423
Select
POLR2F Rabbit anti-Human Polyclonal Antibody
Rat
IF, IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
POLR2F Antibody - Immunofluorescence analysis of HeLa cells.
Select
POLR2F Rabbit anti-Human Polyclonal Antibody
Human
IF, IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
POLR2F Antibody
Select
POLR2F Rabbit anti-Human Polyclonal Antibody
Human
ICC, IF, WB
Unconjugated
50 µl/$286; 100 µl/$321
POLR2F Antibody - 1:100 staining rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
POLR2F Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
Select
POLR2F Rabbit anti-Human Polyclonal Antibody
Human
IF, WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
Select
POLR2F Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
50 µl/$356; 100 µl/$461
Select
POLR2F Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
100 µg/$357
POLR2F Antibody - Anti-POLR2F Antibody (N-Term) at 1:2000 dilution + mouse pancreas lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Select
POLR2F Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Mouse, Human
ELISA, WB
Unconjugated
200 µl/$393
POLR2F Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY POLR2F. (Right lane) cDNA for 48 hrs and lysed
Select
POLR2F Mouse anti-Human Monoclonal (OTI2F2) Antibody
Human
WB
Unconjugated
100 µl/$434
POLR2F Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY POLR2F. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2F. (1:2000)
Select
POLR2F Mouse anti-Human Monoclonal (Carrier-free) (OTI2F2) Antibody
Human
WB
Unconjugated, Carrier-free
100 µg/$511
POLR2F Antibody - Western blot analysis of extracts of mouse pancreas, using POLR2F antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.
Select
POLR2F Rabbit anti-Human Polyclonal Antibody
Mouse, Human
WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
POLR2F Antibody - Western blot analysis of extracts of mouse pancreas using POLR2F Polyclonal Antibody at dilution of 1:3000.
Select
POLR2F Rabbit anti-Human Polyclonal Antibody
Mouse, Human
WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
Select
POLR2F Rabbit anti-Rat Polyclonal Antibody
Rat
WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
POLR2F Protein - Purified recombinant protein POLR2F was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Select
HEK 293 Cells
Myc-DDK (Flag)
14.3 kDa
20 µg/$1,107
POLR2F Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
Select
Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
Select
293T Cells
Myc-DDK (Flag)
14.3 kDa
20 µg/$150
POLR2F Protein - 12.5% SDS-PAGE of human POLR2F stained with Coomassie Blue
Select
Wheat Germ Extract
GST
40.9 kDa
10 µg/$479; 25 µg/$670
POLR2F Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
14.3 kDa
100 µg/$494
POLR2F Protein
Select
E. coli
His
100 µg/$361; 500 µg/$739; 20 µg/$261
Viewing 1-21 of 21 product results



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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).