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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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POLR2A / RNA polymerase II Antibody (phospho‑Ser2/Ser5) LS‑C17711
RNA polymerase II antibody LS-C17711 is an unconjugated rabbit polyclonal antibody to RNA polymerase II (POLR2A) from human, mouse and rat. Validated for IHC and WB.
Antibody
Catalog
Size
Price
LS-C17711-100
100 µl
$575

Popular POLR2A / RNA polymerase II Products

Anti-POLR2A / RNA polymerase II antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6990 dilution 1:100.
Species: Human, Mouse, Rat
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Detection of Human RNA Polymerase II by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg/IP, 20% of IP loaded) from 293T cells. Antibody: Affinity purified rabbit anti-RNA Polymerase II antibody used for WB at 1 ug/ml and used for IP at 6 ug/mg lysate. RNA Polymerase II was also immunoprecipitated by a previous lot of this antibody. Detection: Chemiluminescence with an exposure time of 10 seconds.
Species: Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Xenopus, Zebrafish
Applications: Western blot, Immunoprecipitation, Proximity Ligation Assay
 This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Hamster, Drosophila, Yeast, Plant
Applications: Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Immunofluorescence analysis of HepG2 cells.
Species: Human, Mouse, Rat
Applications: Immunofluorescence, Western blot
Western blot of Rpb1 antibody
Species: Human, Monkey, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot, ELISA

Product Description

POLR2A / RNA polymerase II Antibody (phospho-Ser2/Ser5) for IHC, WB/Western LS-C17711

Specifications

Target
Human POLR2A / RNA polymerase II
Synonyms
POLR2A, HRPB220, HsRPB1, POLR2, RpIILS, RPOL2, RNA Polymerase II, RPO2, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:25)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Ser2/5 of the Rpb1 CTD heptapeptide.
Epitope
pSer2/Ser5
Specificity
Detects endogenous levels of Rpb1 only when the carboxy-terminal domain heptapeptide repeat [Tyr1, Ser2, Pro3, Thr4, Ser5, Pro6, Ser7] is phosphorylated at serine 2/5. Species cross-reactivity: Human, mouse, and rat. Species sequence homology: Hamster.
Usage
Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:1000. Immunohistochemistry (Paraffin): 1:25 (Citrate/TBST).
Presentation
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 100 ug/ml BSA, 50% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About POLR2A / RNA polymerase II
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. P24928 NM_000937 NP_000928.1


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Requested From: United States
Date Requested: 10/15/2018

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