Human POLR2A / RNA polymerase II
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (1:25)
- Western blot (1:1000)
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Synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding Ser2/5 of the Rpb1 CTD heptapeptide.
Detects endogenous levels of Rpb1 only when the carboxy-terminal domain heptapeptide repeat [Tyr1, Ser2, Pro3, Thr4, Ser5, Pro6, Ser7] is phosphorylated at serine 2/5. Species cross-reactivity: Human, mouse, and rat. Species sequence homology: Hamster.
Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:1000. Immunohistochemistry (Paraffin): 1:25 (Citrate/TBST).
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 100 ug/ml BSA, 50% Glycerol
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.