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Antibodies > POLR2A / RNA polymerase II > LS-C287524
 
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Catalog Number Size Price
LS-C287524-10 10 µl $295 
LS-C287524-100 100 µl (0.2 mg/ml) $533 
POLR2A / RNA polymerase II Antibody - Detection of human Phospho-RNA Polymerase II (S2) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) used at a dilution of 1:1000 (0.2µg/ml). Detection: DAB
POLR2A / RNA polymerase II Antibody - Detection of mouse Phospho-RNA Polymerase II (S2) by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) used at a dilution of 1:200 (1µg/ml). Detection: DAB
POLR2A / RNA polymerase II Antibody - Detection of Human Phospho-RNA Polymerase II (S2) by Immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) used at a dilution of 1:500 ( 0.4 ug/ml). Detection: DAB.
POLR2A / RNA polymerase II Antibody - Detection of human Phospho RNA Polymerase II (S2) by western blot. Samples: Whole cell lysate (50 and 15 µg) from HeLa prepared using NETN buffer. Lysate was mock treated (-) or treated with phosphatases (+). Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody was used for WB at 0.04 µg/ml (upper panel). To examine total RNA Polymerase II, rabbit anti-RNA Polymerase II antibody was used at 1 µg/ml (lower panel). Detection: Chemiluminescence with exposure time of 3 seconds.
POLR2A / RNA polymerase II Antibody - Detection of Mouse Phospho RNA Polymerase II (S2) by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from NIH3T3 cells. Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody used for WB at 0.08 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
POLR2A / RNA polymerase II Antibody - Detection of human Phospho-RNA Polymerase II (S2) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) used at a dilution of 1:1000 (0.2µg/ml). Detection: DAB
POLR2A / RNA polymerase II Antibody - Detection of mouse Phospho-RNA Polymerase II (S2) by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) used at a dilution of 1:200 (1µg/ml). Detection: DAB
POLR2A / RNA polymerase II Antibody - Detection of Human Phospho-RNA Polymerase II (S2) by Immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) used at a dilution of 1:500 ( 0.4 ug/ml). Detection: DAB.
POLR2A / RNA polymerase II Antibody - Detection of human Phospho RNA Polymerase II (S2) by western blot. Samples: Whole cell lysate (50 and 15 µg) from HeLa prepared using NETN buffer. Lysate was mock treated (-) or treated with phosphatases (+). Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody was used for WB at 0.04 µg/ml (upper panel). To examine total RNA Polymerase II, rabbit anti-RNA Polymerase II antibody was used at 1 µg/ml (lower panel). Detection: Chemiluminescence with exposure time of 3 seconds.
POLR2A / RNA polymerase II Antibody - Detection of Mouse Phospho RNA Polymerase II (S2) by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from NIH3T3 cells. Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody used for WB at 0.08 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
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Polyclonal Rabbit anti‑Human POLR2A / RNA polymerase II Antibody (phospho‑Ser2, IHC, WB) LS‑C287524

Polyclonal Rabbit anti‑Human POLR2A / RNA polymerase II Antibody (phospho‑Ser2, IHC, WB) LS‑C287524

Submit Review LSBio Guarantee
Antibody:
POLR2A / RNA polymerase II Rabbit anti-Human Polyclonal (pSer2) Antibody
Application:
IHC, WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$295
LS-C287524-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
POLR2A / RNA polymerase II Rabbit anti-Human Polyclonal (pSer2) Antibody
Application:
IHC, WB, IP
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
RNA polymerase II antibody LS-C287524 is an unconjugated rabbit polyclonal antibody to RNA polymerase II (POLR2A) (pSer2) from human. It is reactive with human and mouse. Validated for IHC, IP and WB.
Target
Human POLR2A / RNA polymerase II
Synonyms
POLR2A | HRPB220 | HsRPB1 | POLR2 | RpIILS | RPOL2 | RNA Polymerase II | RPO2 | POLRA | RNA polymerase II subunit B1 | RPB1 | RPBh1
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Drosophila melanogaster (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A portion of GeneID: 5430 corresponding to amino acids surrounding serine 2 of UniprotID: P24928
Epitope
pSer2
Specificity
Human POLR2A / RNA polymerase II (phospho-Ser2)
Applications
  • IHC (1:200 - 1:1000)
  • Western blot (1:2000 - 1:10000)
  • Immunoprecipitation (2 - 10 µg/mg lysate)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Manufacturer
Bethyl Laboratories, Inc.
Presentation
TBS, 0.09% Sodium Azide, 0.1% BSA
Storage
Store at 2-8°C for up to one year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About POLR2A / RNA polymerase II
P24928 NM_000937 NP_000928.1

Publications (0)

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IHC‑plus™ POLR2A / RNA polymerase II Antibody (aa1585‑1634) LS‑B6990
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
POLR2A / RNA polymerase II Antibody - Detection of Human and Mouse RNA Polymerase II by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug for WB), HeLa (H; 50 ug), Jurkat (J; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RNA Polymerase II antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
POLR2A / RNA polymerase II Antibody (C‑Terminus) LS‑C287522
Species: Human, Mouse
Applications: Western blot, Immunoprecipitation
POLR2A / RNA polymerase II Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ POLR2A / RNA polymerase II Antibody (C‑Terminus) LS‑B14574
Species: Human, Monkey, Mouse, Rat, Dog, Zebrafish
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
POLR2A / RNA polymerase II Antibody - This image was taken for the unconjugated form of this product. Other forms have not been tested.
POLR2A / RNA polymerase II Antibody (phospho‑Ser, clone 4H8) LS‑C49196
Species: Human, Mouse, Hamster, Drosophila, Yeast, Plant
Applications: Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Product
POLR2A / RNA polymerase II Antibody LS‑C26239
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Xenopus, Zebrafish, Drosophila, Insect
Applications: IHC, IHC - Frozen, Western blot
POLR2A / RNA polymerase II Antibody - All lanes: Anti-POLR2A Antibody (monoclonal) (M01AA) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HT-29 whole cell lysate Lane 3: CCRF-CEM whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 217 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
POLR2A / RNA polymerase II Antibody (clone 1357CT288.1.37) LS‑C757523
Species: Human
Applications: Western blot, ELISA
See all POLR2A / RNA polymerase II Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
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