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Catalog Number Size Price
LS-B5464-50 50 µg $460 
POLK / DNA Polymerase Kappa Antibody - Anti-POLK antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
POLK / DNA Polymerase Kappa Antibody - Immunofluorescence of monoclonal antibody to POLK on HeLa cell. [antibody concentration 10 ug/ml]
POLK / DNA Polymerase Kappa Antibody - Western blot of POLK expression in transfected 293T cell line by POLK monoclonal antibody, clone 6F2.
POLK / DNA Polymerase Kappa Antibody - Western blot of POLK expression in HeLa nuclear extracts by POLK monoclonal antibody, clone 6F2.
POLK / DNA Polymerase Kappa Antibody - Detection limit for recombinant GST tagged POLK is approximately 0.3 ng/ml as a capture antibody.
POLK / DNA Polymerase Kappa Antibody - Western blot of POLK over-expressed 293 cell line, cotransfected with POLK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with POLK monoclonal antibody, clone 6F2. GAPDH (36.1 kD) used as control.
POLK / DNA Polymerase Kappa Antibody - Anti-POLK antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
POLK / DNA Polymerase Kappa Antibody - Immunofluorescence of monoclonal antibody to POLK on HeLa cell. [antibody concentration 10 ug/ml]
POLK / DNA Polymerase Kappa Antibody - Western blot of POLK expression in transfected 293T cell line by POLK monoclonal antibody, clone 6F2.
POLK / DNA Polymerase Kappa Antibody - Western blot of POLK expression in HeLa nuclear extracts by POLK monoclonal antibody, clone 6F2.
POLK / DNA Polymerase Kappa Antibody - Detection limit for recombinant GST tagged POLK is approximately 0.3 ng/ml as a capture antibody.
POLK / DNA Polymerase Kappa Antibody - Western blot of POLK over-expressed 293 cell line, cotransfected with POLK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with POLK monoclonal antibody, clone 6F2. GAPDH (36.1 kD) used as control.
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IHC‑plus™ Monoclonal Mouse anti‑Human POLK / DNA Polymerase Kappa Antibody (clone 6F2, IHC, IF, WB) LS‑B5464

IHC‑plus™ Monoclonal Mouse anti‑Human POLK / DNA Polymerase Kappa Antibody (clone 6F2, IHC, IF, WB) LS‑B5464

Note: This antibody replaces LS-C133757
Antibody:
POLK / DNA Polymerase Kappa Mouse anti-Human Monoclonal (6F2) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA, RNAi
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B5464-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
POLK / DNA Polymerase Kappa Mouse anti-Human Monoclonal (6F2) Antibody
Application:
IHC, IHC-P, IF, WB, ELISA, RNAi
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
DNA Polymerase Kappa antibody LS-B5464 is an unconjugated mouse monoclonal antibody to human DNA Polymerase Kappa (POLK). Validated for ELISA, IF, IHC, RNAi and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human POLK / DNA Polymerase Kappa
Synonyms
POLK | DINP | DNA polymerase kappa | DINB1 | DINB protein
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
6F2
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Immunogen
POLK (AAH50718, 1 aa ~ 473 aa) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human POLK
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
  • RNA interference
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About POLK / DNA Polymerase Kappa
Q9UBT6 NM_016218 NP_057302.1

Validation

POLK / DNA Polymerase Kappa Antibody - Anti-POLK antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-POLK antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-POLK antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ POLK / DNA Polymerase Kappa Antibody (clone 6F2) for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B5464 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024