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Anti-POLK / DNA Polymerase Kappa Antibody (aa817-830) IHC-plus™ LS-B405

Note: This antibody replaces LS-C19106

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular POLK / DNA Polymerase Kappa Antibodies

Anti-POLK / DNA Polymerase Kappa Antibody (aa817-830) IHC-plus™ LS-B405
Goat Polyclonal to Human POLK / DNA Polymerase Kappa
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-POLK / DNA Polymerase Kappa Antibody (clone 6F2) IHC-plus™ LS-B5464
Mouse Monoclonal [clone 6F2] (IgG1,k) to Human POLK / DNA Polymerase Kappa
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-POLK / DNA Polymerase Kappa Antibody IHC-plus™ LS-B13397
Rabbit Polyclonal (IgG) to Human POLK / DNA Polymerase Kappa
Human, Mouse, Rat
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human POLK / DNA Polymerase Kappa
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human POLK / DNA Polymerase Kappa
Goat
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence
  • Western blot (1:500 - 1:3000)
  • ELISA (1:2000 - 1:10000)

Specificity and Use

POLK / DNA Polymerase Kappa antibody was raised against synthetic peptide from human POLK.
aa817-830
Amino acids 817-830 of Human POLK (polymerase (DNA directed) kappa).
Immunohistochemistry: LS-B405 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B405 was determined to be 5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About POLK / DNA Polymerase Kappa

Q9UBT6 NM_016218 NP_057302.1

POLK Antibody, DINP Antibody, DNA polymerase kappa Antibody, DINB1 Antibody, DINB protein Antibody

DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.

Immunohistochemistry

Immunohistochemistry
Anti-POLK antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B405 concentration 5 ug/ml.

Western blot

Western blot
Anti-POLK Antibody - Western Blot. Western blot of Affinity Purified anti-POLK antibody shows detection of a band ~98 kD corresponding to human POLK (arrowhead). Approximately 35 ug of a HeLa whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000. Reaction occurred overnight at 4? followed by washes and reaction with a 1:20000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Requested From: United States
Date Requested: 12/6/2016

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