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Anti-POLI Antibody (C-Terminus) IHC-plus™ LS-B296

Note: This antibody replaces LS-C2651, LS-C22857

Ordering

Wt. Vol. Conc. Price
- 50 µl - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular POLI Antibodies

Anti-POLI Antibody (C-Terminus) IHC-plus™ LS-B296
Rabbit Polyclonal to Human POLI
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-POLI Antibody (clone 1B9) LS-C175631
Mouse Monoclonal [clone 1B9] (IgG1) to Human POLI
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-POLI Antibody (aa673-701, AP) LS-C284662
Rabbit Polyclonal (IgG) to Human POLI
Human
IHC, Western blot, ELISA
AP Conjugated

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Human POLI
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated

Details

Human POLI
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Antiserum
Unmodified

Applications

  • IHC - Paraffin (1:500)
  • Western blot (1:5000)

Specificity and Use

POLI antibody was raised against a 15-mer peptide in the C-terminal region of human DNA Polymerase Iota, conjugated to KLH
C-Terminus
Human DNA Polymerase Iota.
Immunohistochemistry: LS-B296 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B296 was determined to be 1:500.

Packaging

PBS, 0.02% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

Publications (1)

1
Overexpressed DNA polymerase iota regulated by JNK/c-Jun contributes to hypermutagenesis in bladder cancer. Yuan F, Xu Z, Yang M, Wei Q, Zhang Y, Yu J, Zhi Y, Liu Y, Chen Z, Yang J. PloS one. 2013 8:e69317. [Full Text Article] [PubMed:23922701] [PMC:PMC3724822]

About POLI

Q9UNA4 NM_007195 NP_009126.2

POLI Antibody, DNA polymerase iota Antibody, Eta2 Antibody, Polymerase (DNA directed) iota Antibody, RAD30 homolog B Antibody, RAD3OB Antibody, RAD30B Antibody

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes.

Immunohistochemistry

Immunohistochemistry
Anti-POLI antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B296 dilution 1:500.

Immunohistochemistry

Immunohistochemistry
Anti-POLI antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B296 dilution 1:500.

Requested From: United States
Date Requested: 12/9/2016

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