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POLI

polymerase (DNA directed) iota

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunogobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.

Gene Name: polymerase (DNA directed) iota
Synonyms: POLI, DNA polymerase iota, Eta2, Polymerase (DNA directed) iota, RAD30 homolog B, RAD3OB, RAD30B
Target Sequences: NM_007195 NP_009126.2 Q9UNA4

Publications (3)

1
DNA polymerase zeta cooperates with polymerases kappa and iota in translesion DNA synthesis across pyrimidine photodimers in cells from XPV patients. Ziv O, Geacintov N, Nakajima S, Yasui A, Livneh Z. Proceedings of the National Academy of Sciences of the United States of America. 2009 106:11552-7. [PubMed:19564618] [PMC:PMC2710681]
2
DNA damage bypass operates in the S and G2 phases of the cell cycle and exhibits differential mutagenicity. Diamant N, Hendel A, Vered I, Carell T, Reissner T, de Wind N, Geacinov N, Livneh Z. Nucleic acids research. 2012 40:170-80. [PubMed:21908406] [PMC:PMC3245908]
3
Overexpressed DNA polymerase iota regulated by JNK/c-Jun contributes to hypermutagenesis in bladder cancer. Yuan F, Xu Z, Yang M, Wei Q, Zhang Y, Yu J, Zhi Y, Liu Y, Chen Z, Yang J. PloS one. 2013 8:e69317. [Full Text Article] [PubMed:23922701] [PMC:PMC3724822] Related Antibodies: LS-B296.

☰ Filters
Antibodies (46)
Proteins (5)
Over-Expression Lysate (2)
Primary (46)
Recombinant (3)
POLI (51)
Human (46)
Mouse (7)
Rat (5)
Bat (3)
Bovine (3)
Dog (3)
Gibbon (3)
Guinea pig (3)
Horse (3)
Monkey (4)
Pig (3)
Zebrafish (3)
IHC (17)
IHC-P (6)
WB (41)
ELISA (10)
IF (2)
IP (3)
Peptide-ELISA (3)
rabbit (28)
mouse (18)
Product Group
IHCPlus (1)
IgG (20)
IgG1 (18)
monoclonal mc (18)
polyclonal pc (28)
OTI1B9 (2)
OTI1C8 (2)
OTI1H9 (2)
OTI2C10 (2)
OTI3B5 (2)
OTI4C12 (2)
OTI5A4 (2)
OTI5D11 (2)
OTI5H9 (2)
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Azide-free (1)
Biotin Conjugated (3)
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FITC Conjugated (3)
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Unconjugated (35)
aa458-740 (11)
aa673-701 (7)
aa143-192 (4)
610-690 aa, C-terminal (1)
C-Terminus (1)
aa626-675 (1)
aa640-690 (1)
aa641-690 (1)
aa690-715 (1)
No (50)
Yes (1)
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Human (4)
293T Cells (1)
HEK 293 Cells (2)
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Purified (1)
POLI Antibody - Anti-POLI antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:500.
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POLI Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Mouse, Human
IHC, IHC-P, WB
Unconjugated
0.05 ml/$460
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POLI Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IHC, IP, WB
Unconjugated
100 µg/$331
POLI Antibody - IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-POLI mouse monoclonal antibody.
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POLI Mouse anti-Human Monoclonal (OTI1B9) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$434
POLI Antibody - IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-POLI mouse monoclonal antibody.
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POLI Mouse anti-Human Monoclonal (aa458-740) (Carrier-free) (OTI1B9) Antibody
Human
IHC, WB
Unconjugated, Carrier-free
100 µg/$511
POLI Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLI (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLI.
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POLI Mouse anti-Human Monoclonal (OTI1H9) Antibody
Human
WB
Unconjugated
100 µl/$434
POLI Antibody - Immunohistochemistry image of paraffin-embedded human testis tissue at a dilution of 1:100
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POLI Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, IHC-P
Unconjugated
50 µg/$294; 100 µg/$360
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POLI Rabbit anti-Human Polyclonal (aa673-701) Antibody
Human
ELISA, IHC, WB
Unconjugated
200 µl/$734
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POLI Rabbit anti-Human Polyclonal (aa673-701) (Biotin) Antibody
Human
ELISA, IHC, WB
Biotin Conjugated
200 µl/$952
Select
POLI Rabbit anti-Human Polyclonal (aa673-701) (FITC) Antibody
Human
ELISA, IHC, WB
FITC Conjugated
200 µl/$952
Select
POLI Rabbit anti-Human Polyclonal (aa673-701) (APC) Antibody
Human
ELISA, IHC, WB
APC Conjugated
200 µl/$952
Select
POLI Rabbit anti-Human Polyclonal (aa673-701) (PE) Antibody
Human
ELISA, IHC, WB
PE Conjugated
200 µl/$952
Select
POLI Rabbit anti-Human Polyclonal (aa673-701) (Azide-free) (HRP) Antibody
Human
ELISA, IHC, WB
HRP Conjugated, Azide-free
200 µl/$952
POLI Antibody - IHC of paraffin-embedded Carcinoma of Human thyroid tissue using anti-POLI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
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POLI Mouse anti-Human Monoclonal (aa458-740) (OTI1C8) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$434
POLI Antibody - IHC of paraffin-embedded Carcinoma of Human thyroid tissue using anti-POLI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
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POLI Mouse anti-Human Monoclonal (aa458-740) (Carrier-free) (OTI1C8) Antibody
Human
IHC, WB
Unconjugated, Carrier-free
100 µg/$511
POLI Antibody - IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-POLI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
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POLI Mouse anti-Human Monoclonal (aa458-740) (OTI3B5) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$434
POLI Antibody - IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-POLI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
Select
POLI Mouse anti-Human Monoclonal (aa458-740) (Carrier-free) (OTI3B5) Antibody
Human
IHC, WB
Unconjugated, Carrier-free
100 µg/$511
POLI Antibody - Staining MCF-7 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
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POLI Rabbit anti-Human Polyclonal Antibody
Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
POLI Antibody - POLI Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
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POLI Rabbit anti-Human Polyclonal (aa673-701) Antibody
Human
IHC, IHC-P, WB
Unconjugated
400 µl/$393
POLI Antibody - Western blot analysis of extracts of various cell lines.
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POLI Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
POLI Antibody - Western blot of DNA pol iota antibody
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POLI Rabbit anti-Human Polyclonal (610-690 aa, C-terminal) Antibody
Human
ELISA, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
POLI Antibody - Western blot analysis of extracts from HeLa cells, using POLI antibody.
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POLI Rabbit anti-Human Polyclonal Antibody
Human
ELISA, WB
Unconjugated
100 µg/$357
Select
POLI Rabbit anti-Human Polyclonal (aa690-715) Antibody
Human
IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
POLI Antibody - Western blot analysis of lysates from HeLa cells, using POLI Antibody. The lane on the right is blocked with the synthesized peptide.
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POLI Rabbit anti-Human Polyclonal (aa641-690) Antibody
Human
Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
POLI Antibody - Western blot analysis of the lysates from HeLa cells using POLI antibody.
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POLI Rabbit anti-Human Polyclonal (aa626-675) Antibody
Human
Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
POLI Antibody - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLI (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLI.
Select
POLI Mouse anti-Human Monoclonal (OTI5H9) Antibody
Human
WB
Unconjugated
100 µl/$434
Viewing 1-25 of 51 product results



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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).