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Anti-POLD1 Antibody (aa1-140, clone 607) IHC-plus™ LS-B1325

Note: This antibody replaces LS-C19968
Catalog Size Price
LS-B1325-50 50 µl (1 mg/ml) Unavailable

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19 POLD1 Antibodies

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Anti-POLD1 Antibody (aa1-140, clone 607) IHC-plus™ LS-B1325
Mouse Monoclonal [clone 607] (IgG) to Human POLD1
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-POLD1 Antibody (clone 2761C6a) LS-C184547
Mouse Monoclonal [clone 2761C6a] (IgG2a) to Human POLD1
Human, Rat
ICC, Western blot, Dot Blot
Unconjugated
Western blot Image
Anti-POLD1 Antibody (aa685-1071) LS-C185507
Rabbit Polyclonal to Human POLD1
Human
ICC, Western blot
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Anti-POLD1 Antibody LS-C333982
Rabbit Polyclonal (IgG) to Human POLD1
Human, Mouse, Rat
IHC, Immunofluorescence, Western blot
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Immunofluorescence Image
Anti-POLD1 Antibody (clone 2761C6a) LS-C342585
Mouse Monoclonal [clone 2761C6a] (IgG2a) to Human POLD1
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ICC, Immunofluorescence, Western blot, Dot Blot
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Immunofluorescence Image

100% Guaranteed
Mouse Monoclonal [clone 607] (IgG) to Human POLD1
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human POLD1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG Monoclonal [607]
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot

Specificity and Use

POLD1 antibody was raised against recombinant human POLD1.
aa1-140
Recombinant protein encoding amino acids 1-140 of the large subunit of human DNA polymerase d.
Immunohistochemistry: LS-B1325 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1325 was determined to be 10 ug/ml.

Packaging

PBS. Sourced in TCS.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About POLD1

P28340 NM_002691 NP_002682.2

POLD1 Antibody, CDC2 Antibody, CDC2 homolog Antibody, POLD Antibody

Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex.

Immunohistochemistry

Immunohistochemistry
Anti-POLD1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1325 concentration 10 ug/ml.

Requested From: 
Date Requested: 8/19/2017

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