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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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POLB / DNA Polymerase Beta Antibody (clone 18S) LS‑C88354
Note: This antibody replaces LS-C22846
Antibody
DNA Polymerase Beta antibody LS-C88354 is an unconjugated mouse monoclonal antibody to DNA Polymerase Beta (POLB) from human, mouse, rat and other species. Validated for IP and WB.
Catalog
Size
Price
LS-C88354-0.1
0.1 ml
Unavailable
LS-C88354-0.5
0.5 ml
Unavailable
LS-C88354-1
1 ml
Unavailable

Popular POLB / DNA Polymerase Beta Products

Antibody
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Anti-POLB / DNA Polymerase Beta antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7021 dilution 1:200.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Western blot of Rabbit Anti-POL (DNA polymerase beta) Antibody (LS-C153861). Lane 1: LN428 FLAG POL (control). Lane 2: A172. Load: 35 ug per lane. Primary antibody: POL antibody at 1:2000 for overnight at 4C. Secondary antibody: IRDye800 goat anti-rabbit at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: ~40 kDa, ~40 kDa. Other band(s): unknown band ~75 kDd.
Species: Human
Applications: Western blot, ELISA
Immunohistochemistry of paraffin-embedded human esophageal tissue.
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
Antibody
Species: Human
Applications: IHC, Western blot, Immunoprecipitation, ELISA

Product Description

DNA Polymerase Beta antibody LS-C88354 is an unconjugated mouse monoclonal antibody to DNA Polymerase Beta (POLB) from human, mouse, rat and other species. Validated for IP and WB.
About POLB / DNA Polymerase Beta
Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. P06746 NM_002690 NP_002681.1

POLB Antibody, DNA pol beta Antibody, Mutant DNA polymerase beta Antibody, DNA polymerase beta Antibody, DNA polymerase beta subunit Antibody


Specifications

Target
Rat POLB / DNA Polymerase Beta
Host
Mouse
Reactivity
Rat, Human, Mouse, Bovine, Hamster, Xenopus (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [18S]
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Protein G purified
Modifications
Unmodified. Also available Azide-free.
Applications
  • Western blot (1 - 2 µg/ml)
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Immunogen
Rat DNA polymerase beta protein.
Presentation
10mM PBS, pH 7.4, with 0.2% BSA and 0.09% Sodium Azide
Storage
Stable for 2 years when stored at 2-8°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/18/2018

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