• Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA and Assay Kits
  • All Kits
  • Assay Kits
  • Traditional ELISA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Phospho-Specific ELISA Kits
  • ELISA Development Kits
  • Chemiluminescent CLIA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Biochemicals
  • All Biochemicals

  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question?
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Protocols
  • Secure Logins
  • Login to Our Secure ShareFile Site
  • Login to Our IHC Database
Contact Us

2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products. - To submit a new order. - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues. - To request technical information requests about an LSBio product or its application. - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login ▾
Wish List
View Cart

Anti-POFUT1 Antibody (aa81-95) LS-C186579

Catalog Size Price
LS-C186579-100 100 µg (0.5 mg/ml) Unavailable

Related Products

24 POFUT1 Antibodies

Most Popular POFUT1 Antibodies

Anti-POFUT1 Antibody (aa17-180) LS-C179093
Rabbit Polyclonal to Human POFUT1
IHC, Western blot, ELISA
Immunohistochemistry Image
Anti-POFUT1 Antibody (aa351-381) LS-C324154
Rabbit Polyclonal (IgG) to Human POFUT1
IHC, Western blot, ELISA
Anti-POFUT1 Antibody (C-Terminus) LS-C353355
Rabbit Polyclonal to Human POFUT1
Human, Monkey, Dog, Pig
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
Immunohistochemistry Image
Anti-POFUT1 Antibody (aa300-380) LS-C385014
Rabbit Polyclonal (IgG) to Human POFUT1
Human, Mouse, Rat
IHC, Western blot, ELISA
Anti-POFUT1 Antibody (aa243-393, FITC) LS-C421580
Rabbit Polyclonal (IgG) to Mouse POFUT1
ICC, Western blot, ELISA
FITC Conjugated

100% Guaranteed
Goat Polyclonal to Human POFUT1
Human, Dog, Hamster, Horse, Pig
IHC - Paraffin, ELISA


Human POFUT1
Human, Dog, Hamster, Horse, Pig (tested or 100% immunogen sequence identity)
Bovine, Guinea pig (at least 90% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Paraffin
  • ELISA (1:16000)

Specificity and Use

POFUT1 antibody was raised against synthetic peptide C-TNLHVSYQKYFKLEP from an internal region of human POFUT1 (NP_056167.1; NP_758436.1). Percent identity by BLAST analysis: Hamster, Elephant, Panda, Dog, Cat, Horse, Pig (100%); Bovine, Guinea pig (93%); Opossum, Turkey, Chicken (80%).
LS-E27896 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C186579. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human POFUT1. This antibody is expected to recognize both reported isoforms (NP_056167.1; NP_758436.1).
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: In transfected HEK293 transiently expressing Human POFUT1 (myc and DYKDDDDK tagged), a band of approx. 48kD is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration, 0.3-1 ug/ml. IHC: In paraffin embedded Human Cerebral Cortex shows staining of the cytoplasm in neuronal bodies. Recommended concentration, 2-4 ug/ml.


Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About POFUT1

Q9H488 D80002 Q9H488

POFUT1 Antibody, KIAA0180 Antibody, FUT12 Antibody, O-Fuc-T Antibody, Protein O-fucosyltransferase 1 Antibody, O-fucosyltransferase protein Antibody, O-FucT-1 Antibody, O-FUT Antibody, Peptide-O-fucosyltransferase 1 Antibody

Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling.


POFUT1 antibody (2 ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Western blot

Western blot
POFUT1 antibody HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human POFUT1 with C-terminal MYC tag probed with (1 ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with (1 mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Requested From: 
Date Requested: 8/22/2017

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2017 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number