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Anti-POFUT1 Antibody (aa81-95) LS-C186579


Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml $385
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular POFUT1 Antibodies

Anti-POFUT1 Antibody (N-Terminus) LS-C83909
Rabbit Polyclonal to Human POFUT1
Human, Mouse
Western blot
Western blot Image
Anti-POFUT1 Antibody (aa17-180) LS-C179093
Rabbit Polyclonal to Human POFUT1
IHC, Western blot, ELISA
Immunohistochemistry Image
Anti-POFUT1 Antibody (aa344-373, PE) LS-C266686
Rabbit Polyclonal (IgG) to Human POFUT1
IHC, Western blot, ELISA
PE Conjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human POFUT1
Human, Dog, Hamster, Horse, Pig
IHC - Paraffin, ELISA


Human POFUT1
Human, Dog, Hamster, Horse, Pig (tested or 100% immunogen sequence identity)
Bovine, Guinea pig (at least 90% immunogen sequence identity)
Immunoaffinity purified


  • IHC - Paraffin
  • ELISA (1:16000)

Specificity and Use

POFUT1 antibody was raised against synthetic peptide C-TNLHVSYQKYFKLEP from an internal region of human POFUT1 (NP_056167.1; NP_758436.1). Percent identity by BLAST analysis: Hamster, Elephant, Panda, Dog, Cat, Horse, Pig (100%); Bovine, Guinea pig (93%); Opossum, Turkey, Chicken (80%).
LS-E27896 - Lyophilized - 100 µg - $145.00
Human POFUT1. This antibody is expected to recognize both reported isoforms (NP_056167.1; NP_758436.1).
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: In transfected HEK293 transiently expressing Human POFUT1 (myc and DYKDDDDK tagged), a band of approx. 48kD is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration, 0.3-1 ug/ml. IHC: In paraffin embedded Human Cerebral Cortex shows staining of the cytoplasm in neuronal bodies. Recommended concentration, 2-4 ug/ml.


Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About POFUT1

Q9H488 D80002 Q9H488

POFUT1 Antibody, KIAA0180 Antibody, FUT12 Antibody, O-Fuc-T Antibody, Protein O-fucosyltransferase 1 Antibody, O-fucosyltransferase protein Antibody, O-FucT-1 Antibody, O-FUT Antibody, Peptide-O-fucosyltransferase 1 Antibody

Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling.


POFUT1 antibody (2 ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Western blot

Western blot
POFUT1 antibody HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human POFUT1 with C-terminal MYC tag probed with (1 ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with (1 mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 10/21/2016

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