Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Human PODXL / Podocalyxin
Human (tested or 100% immunogen sequence identity)
IHC - Paraffin (5 µg/ml)
Western blot (2 µg/ml)
(applications tested for the base form of this product only)
Specificity and Use
PODXL / Podocalyxin antibody was raised against a synthetic peptide within an internal region (residues 350-450) of the human Podocalyxin protein. [Swiss-Prot# O00592] Percent identity by BLAST analysis: Dog (80%); Rat (76%); Mouse (70%).
Podocalyxin - Hematopoietic Stem Cell Marker.
Tris-glycine, 150 mM sodium chloride, 0.05% sodium azide.
Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli.
Anti-PODXL antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5362 concentration 5 ug/ml. This image was taken for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested.
Detection of podocalyxin in human lung lysate. This image was taken for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested.
Requested From: United States
Date Requested: 10/25/2016