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Anti-PNKP Antibody (Internal) IHC-plus™ LS-B404

Note: This antibody replaces LS-C18998

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PNKP Antibodies

Anti-PNKP Antibody (Internal) IHC-plus™ LS-B404
Rabbit Polyclonal to Human PNKP
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-PNKP Antibody (aa109-138, APC) LS-C266631
Rabbit Polyclonal (IgG) to Human PNKP
Human
Western blot, ELISA
APC Conjugated
Anti-PNKP Antibody (aa109-138, Biotin) LS-C266632
Rabbit Polyclonal (IgG) to Human PNKP
Human
Western blot, ELISA
Biotin Conjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human PNKP
Human
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human PNKP
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence
  • Western blot (1:300 - 1:1500)
  • ELISA (1:3000 - 1:12000)

Specificity and Use

PNKP antibody was raised against synthetic peptide from human PNKP.
Internal
An internal region of human PNK protein.
Immunohistochemistry: LS-B404 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B404 was determined to be 5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: -20°C
For research use only.

About PNKP

Q96T60 NM_007254 NP_009185.2

PNKP Antibody, EIEE10 Antibody, DNA 5-kinase/3-phosphatase Antibody, PNK Antibody, MCSZ Antibody

Plays a key role in the repair of DNA damage, functioning as part of both the non-homologous end-joining (NHEJ) and base excision repair (BER) pathways. Through its two catalytic activities, PNK ensures that DNA termini are compatible with extension and ligation by either removing 3'-phosphates from, or by phosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNA backbone.

Immunohistochemistry

Immunohistochemistry
Anti-PNKP antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B404 concentration 5 ug/ml.

Western blot

Western blot
Anti-PNK Antibody - Western Blot. Western blot of Affinity Purified anti-PNK antibody shows detection of a 57 kD band corresponding to human PNK in a Y190 yeast cell lysate (lane 1), Y190 yeast cell lysate + human PNK (Gal DNA BP) (lane 2), EM9 XH Chinese hamster ovary cell lysate (lane 3), EM9 XH Chinese hamster ovary cell lysate + human PNK (lane 4) and a HeLa cell lysate (lane 5). Approximately 10 ug of lysate was run on SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:1000 dilution of anti-PNK antibody incubated for 1 hr in TBST at room temperature. Signal was detected using standard techniques.

Requested From: United States
Date Requested: 12/7/2016

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