Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 1E5] (IgG1) to Human PMEL / SILV / gp100
Mouse Monoclonal (IgG2b) to Human PMEL / SILV / gp100
IHC - Frozen
Human PMEL / SILV / gp100
Human (tested or 100% immunogen sequence identity)
Protein G purified
IHC - Frozen
Specificity and Use
Melanoma Associated Antigen (100/7).
Recognizes a (pre) melanosomal 100+7kD antigen (glycoprotein). Reacts with melanomas, clear cell sarcomas (melanoma of soft tissue), nevocellular nevi and normal melanocytes. Except for one case of non-Hodgkin's lymphoma in which macrophages were positive, no reactions with other tumors or tissues have been observed.
Suitable for use in Immunohistochemistry (Immunoperoxidase staining on frozen tissue sections). Immunohistochemistry: 1:5-1:20 with PBS. Formalin fixation and embedding in paraffin may affect the reactivity of the antibody. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
PBS, pH 7.4. No stabilizing proteins added. No preservative added.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C.
PMEL Antibody, gp100 Antibody, Melanocyte protein PMEL Antibody, Me20m/me20s Antibody, p100 Antibody, PMEL17 Antibody, Premelanosome protein Antibody, SIL Antibody, Silver homolog (mouse) Antibody, Silver, mouse, homolog of Antibody, Silver locus protein homolog Antibody, ME20-M Antibody, Me20-m/me20-s Antibody, ME20M Antibody, Melanocyte protein mel 17 Antibody, Melanocyte protein Pmel 17 Antibody, Silver (mouse homolog) like Antibody, D12S53E Antibody, ME20 Antibody, Melanosomal matrix protein17 Antibody, SILV Antibody
PMEL / SILV / gp100 is a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes.