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Anti-PME-1 / PPME1 Antibody (clone 7F12) LS-C114897

Ordering

Wt. Vol. Conc. Price
- 100 µl - $350
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PME-1 / PPME1 Antibodies

Anti-PME-1 / PPME1 Antibody (aa35-84) IHC-plus™ LS-B6636
Rabbit Polyclonal (IgG) to Human PME-1 / PPME1
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PME-1 / PPME1 Antibody (aa351-379, AP) LS-C284706
Rabbit Polyclonal (IgG) to Human PME-1 / PPME1
Human
Western blot, ELISA
AP Conjugated
Anti-PME-1 / PPME1 Antibody (aa351-379, FITC) LS-C284710
Rabbit Polyclonal (IgG) to Human PME-1 / PPME1
Human
Western blot, ELISA
FITC Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 7F12] (IgG1) to Human PME-1 / PPME1
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human PME-1 / PPME1
Mouse
IgG1 Monoclonal [7F12]
Unconjugated
Affinity purified
Unmodified

Applications

  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)

Specificity and Use

PME-1 / PPME1 antibody was raised against full length human recombinant protein of human PPME1 (NP_057231) produced in HEK293T cell
Human PPME1
Concentration: 0.5-1.0 mg/ml (Lot dependent)

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About PME-1 / PPME1

Q9Y570 NM_016147 NP_057231.1

PPME1 Antibody, PME-1 Antibody, PME1 Antibody

Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro) and PPP2CA. Binding to PPP2CA displaces the manganese ion and inactivates the enzyme.

Immunohistochemistry

Immunohistochemistry
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-PPME1 mouse monoclonal antibody. (Dilution 1:50).

Immunofluorescence

Immunofluorescence
Anti-PPME1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPME1.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPME1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPME1.

Flow Cytometry

Flow Cytometry
Flow cytometric analysis of Jurkat cells, using anti-PPME1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow Cytometry
Flow cytometric analysis of Hela cells, using anti-PPME1 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
 

Requested From: United States
Date Requested: 12/4/2016

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