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Anti-PLXNB3 / Plexin B3 Antibody LS-C150230

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
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Most Popular PLXNB3 / Plexin B3 Antibodies

Anti-PLXNB3 / Plexin B3 Antibody LS-C150230
Sheep Polyclonal (IgG) to Mouse PLXNB3 / Plexin B3
Mouse, Rat
IHC, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-PLXNB3 / Plexin B3 Antibody (aa985-1034) LS-C151964
Rabbit Polyclonal to Human PLXNB3 / Plexin B3
Human
Western blot
Unconjugated
Western blot Image
Anti-PLXNB3 / Plexin B3 Antibody (aa985-1034, FITC) LS-C473056
Rabbit Polyclonal to Human PLXNB3 / Plexin B3
Human
Western blot
FITC Conjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Sheep Polyclonal (IgG) to Mouse PLXNB3 / Plexin B3
Mouse, Rat
IHC, Western blot, Flow Cytometry
Unconjugated

Details

Mouse PLXNB3 / Plexin B3
Sheep
Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC (5 - 15 µg/ml)
  • Western blot (1 µg/ml)
  • Flow Cytometry

Specificity and Use

PLXNB3 / Plexin B3 antibody was raised against chinese hamster ovary cell line CHO-derived recombinant mouse Plexin B3 H is25-Gln 1235 Accession # Q9QY40.
Detects mouse and rat Plexin B3 in direct ELISAs and Western blots. In direct ELISAs, approximately 38% cross-reactivity with recombinant.
Western Blot, 1 µg/ml. Flow Cytometry, 2.5 µg/106 cells. Immunohistochemistry, 5-15 µg/ml.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About PLXNB3 / Plexin B3

Q9ULL4 NM_005393 NP_005384.2

PLXNB3 Antibody, Plexin 6 Antibody, Plexin B3 Antibody, KIAA1206 Antibody, PLEXB3 Antibody, Plexin-B3 Antibody, PLEXR Antibody, PLXN6 Antibody

Receptor for SEMA5A that plays a role in axon guidance, invasive growth and cell migration. Stimulates neurite outgrowth and mediates Ca2+/Mg2+-dependent cell aggregation. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1.

Immunohistochemistry

Immunohistochemistry
Plexin B3 in Mouse Brain. Plexin B3 was detected in perfusion fixed frozen sections of mouse brain using Sheep Anti-Mouse/Rat Plexin B3 Antigen AffinitypurifiedPolyclonalAntibody at 1.7 ug/ml overnight at 4 °C. Tissue was stained using the NorthernLights 557conjugatedAntiSheepIgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to oligodendrocytes.

Western blot

Western blot
Detection of Mouse Plexin B3 by Western Blot. Western blot shows lysates of mouse brain (thalamus/hypothalamus) tissue. PVDF membrane was probed with 1 ug/ml of Sheep Anti-Mouse/Rat Plexin B3 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for Plexin B3 at approximately 180-190 kD (as indicated). This experiment was conducted under reducing conditions.

Flow Cytometry

Flow Cytometry
Detection of Plexin B3 in C6 Rat Cell Line by Flow Cytometry. C6 rat glioma cell line was stained with Sheep Anti-Mouse/Rat Plexin B3 Antigen Affinity-purified Polyclonal Antibody or isotype control antibody, followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody.

Flow Cytometry

Flow Cytometry
Plexin B3 in Mouse Brain. Plexin B3 was detected in perfusion fixed frozen sections of mouse brain using Sheep Anti-Mouse/Rat Plexin B3 Antigen Affinity-purified Polyclonal Antibody at 1.7 ug/ml overnight at 4°C. Tissue was stained using NorthernLights 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to oligodendrocytes.

Requested From: United States
Date Requested: 12/4/2016

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