Dog PLN / Phospholamban
Dog, Human, Bovine, Pig
(tested or 100% immunogen sequence identity)
Monkey, Mouse, Rat, Bat, Horse, Rabbit (at least 90% immunogen sequence identity)
Protein A purified
- IHC - Paraffin (5 µg/ml)
- Western blot
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Synthetic peptide corresponding to aa 2-25 (D2KVQYLTRSAIRRASTIEMPQQAR25) of dog PLB (phospholamban). Percent identity by BLAST analysis: Bovine, Dog, Pig (100%); Human, Monkey, Marmoset, Mouse, Rat, Panda, Bat, Horse, Rabbit, Opossum (96%); Gorilla, Gibbon (92%); Elephant (88%).
Recognizes canine PLB. Recognizes an epitope between aa 9-17 of canine PLB. Detects bands of ~25kD (non-reducing conditions; representing the pentameric form of PLB) and ~5kD (reducing conditions; representing the monomeric form of PLB) by Western Blot. Species cross-reactivity: human, mouse, rat, rabbit chicken, sheep, porcine and guinea pig.
Immunohistochemistry: LS-B2358 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2358 was determined to be 5 ug/ml. Western Blot: 2 ug/ml Detects bands of ~25kD (non-reducing conditions; representing the pentameric form of PLB) and ~5kD (reducing conditions; representing the monomeric form of PLB). Function: Increases the calcium uptake in ventricular SR vesicles.
PBS, 0.05% sodium azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
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