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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PLK1 / PLK‑1 Antibody LS‑C9649
PLK-1 antibody LS-C9649 is an unconjugated mouse monoclonal antibody to human PLK-1 (PLK1). Validated for ELISA, IF, IP and WB.
Antibody
Catalog
Size
Price
LS-C9649-100
100 µg
$605

Popular PLK1 / PLK-1 Products

Anti-PLK1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4225 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Anti-PLK1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1675 concentration 15 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Anti-PLK1 antibody IHC of human lung carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B50 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-PLK1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1674 dilution 1:50.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Anti-PLK1 antibody IHC of human colon, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6646 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot

Product Description

PLK-1 antibody LS-C9649 is an unconjugated mouse monoclonal antibody to human PLK-1 (PLK1). Validated for ELISA, IF, IP and WB.
About PLK1 / PLK-1
Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis. P53350 NM_005030 NP_005021.2

PLK1 Antibody, Polo like kinase Antibody, Polo-like kinase (Drosophila) Antibody, Polo-like kinase 1 Antibody, STPK13 Antibody, Polo (Drosophia)-like kinase Antibody, PLK Antibody, PLK-1 Antibody


Specifications

Target
Human PLK1 / PLK-1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Immunofluorescence
  • Western blot (1 - 3 µg/ml)
  • Immunoprecipitation
  • ELISA (0.1 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
PLK1 / PLK-1 antibody was raised against full-length human Plk1 (polo-like kinase 1), which shares 93-94% amino acid sequence homology with mouse and rat Plk1.
Specificity
Specific for human Plk1. On Western blots, it identifies a band at ~63kD. Reactivity has been confirmed with human HeLa S3 and U-2 OS cells by Western blotting and immunofluorescence, with human HeLa S3 and HEK293 cells by immunoprecipitation, and with mouse NIH3T3 and rat NRK cells by immunofluorescence and Western blotting.
Usage
Suitable for use in ELISA, Immunoprecipitation, Immunofluorescence and Western Blot. ELISA: 0.1-1 ug/ml. Immunoprecipitation: 5-10 ug/test. Immunofluorescence: 0.5-2 ug/test. Western Blot: 1-3 ug/ml.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.

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Requested From: United States
Date Requested: 9/21/2018

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