Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
PLK1 / PLK-1 antibody was raised against full-length human Plk1 (polo-like kinase 1), which shares 93-94% amino acid sequence homology with mouse and rat Plk1.
Specific for human Plk1. On Western blots, it identifies a band at ~63kD. Reactivity has been confirmed with human HeLa S3 and U-2 OS cells by Western blotting and immunofluorescence, with human HeLa S3 and HEK293 cells by immunoprecipitation, and with mouse NIH3T3 and rat NRK cells by immunofluorescence.
Suitable for use in ELISA, Immunoprecipitation, Immunofluorescence and Western Blot. ELISA: 0.1-1 ug/ml. Immunoprecipitation: 5-10 ug/test. Immunofluorescence: 0.5-2 ug/test. Western Blot: 2-3 ug/ml.
PBS, pH 7.4, 0.1% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis.