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Anti-PLK1 / PLK-1 Antibody (clone 8C12) LS-C114499


Wt. Vol. Conc. Price
- 100 µl - $350
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular PLK1 / PLK-1 Antibodies

Anti-PLK1 / PLK-1 Antibody (phospho-Thr210) IHC-plus™ LS-B50
Rabbit Polyclonal to Human PLK1 / PLK-1
IHC - Paraffin, Western blot, ELISA
Immunohistochemistry Image
Anti-PLK1 / PLK-1 Antibody IHC-plus™ LS-B4225
Rabbit Polyclonal to Human PLK1 / PLK-1
IHC - Paraffin, Western blot
Immunohistochemistry Image
Anti-PLK1 / PLK-1 Antibody (aa574-603, Biotin) LS-C266558
Rabbit Polyclonal (IgG) to Human PLK1 / PLK-1
IHC, Western blot, ELISA
Biotin Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 8C12] (IgG1) to Human PLK1 / PLK-1
Human, Monkey, Dog
Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry


Human PLK1 / PLK-1
Human, Monkey, Dog (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [8C12]
Protein A/G purified


  • Immunofluorescence (1:100)
  • Western blot (1:1000 - 1:2000)
  • Immunoprecipitation
  • Flow Cytometry (1:100)

Specificity and Use

PLK1 / PLK-1 antibody was raised against protein expressed in 293T cell transfected with human PLK1 expression vector.
Human PLK / PLK1
Concentration: 0.5-1.0 mg/ml (Lot dependent)


PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About PLK1 / PLK-1

P53350 NM_005030 NP_005021.2

PLK1 Antibody, Polo like kinase Antibody, Polo-like kinase (Drosophila) Antibody, Polo-like kinase 1 Antibody, STPK13 Antibody, Polo (Drosophia)-like kinase Antibody, PLK Antibody, PLK-1 Antibody

Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis.


Anti-PLK1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PLK1.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK1.

Western blot

Western blot
Western blot of extracts (35 ug) from 9 different cell lines by using anti-PLK1 monoclonal antibody.


Immunoprecipitation(IP) of PLK1 by using monoclonal anti-PLK1 antibodies (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-PLK1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Flow Cytometry

Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PLK1 antibody, and then analyzed by flow cytometry.

Requested From: United States
Date Requested: 10/27/2016

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