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Anti-PLK1 / PLK-1 Antibody (clone 2G12) LS-C197924

Ordering

Wt. Vol. Conc. Price
100 µg - - $355
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PLK1 / PLK-1 Antibodies

Anti-PLK1 / PLK-1 Antibody (phospho-Thr210) IHC-plus™ LS-B50
Rabbit Polyclonal to Human PLK1 / PLK-1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-PLK1 / PLK-1 Antibody IHC-plus™ LS-B4225
Rabbit Polyclonal to Human PLK1 / PLK-1
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PLK1 / PLK-1 Antibody (aa574-603, Biotin) LS-C266558
Rabbit Polyclonal (IgG) to Human PLK1 / PLK-1
Human
IHC, Western blot, ELISA
Biotin Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 2G12] (IgG1,k) to Human PLK1 / PLK-1
Human
Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human PLK1 / PLK-1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [2G12]
Unconjugated
Protein A purified
Unmodified

Applications

  • Immunofluorescence (10 µg/ml)
  • Western blot
  • Immunoprecipitation
  • ELISA

Specificity and Use

PLK1 / PLK-1 antibody was raised against pLK1 (AAH02369, aa1-603 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Human PLK1 / PLK-1
Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Cell lysate, Transfected lysate, Recombinant protein.

Packaging

PBS, pH 7.2
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
For research use only.

About PLK1 / PLK-1

P53350 NM_005030 NP_005021.2

PLK1 Antibody, Polo like kinase Antibody, Polo-like kinase (Drosophila) Antibody, Polo-like kinase 1 Antibody, STPK13 Antibody, Polo (Drosophia)-like kinase Antibody, PLK Antibody, PLK-1 Antibody

Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis.

Immunofluorescence

Immunofluorescence
Immunofluorescence of monoclonal antibody to PLK1 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

Western blot
Western Blot analysis of PLK1 expression in transfected 293T cell line by PLK1 monoclonal antibody (M01), clone 2G12.Lane 1: PLK1 transfected lysate(68.3 KDa).Lane 2: Non-transfected lysate.

Western blot

Western blot
PLK1 monoclonal antibody (M01), clone 2G12 Western Blot analysis of PLK1 expression in HeLa NE.

Western blot

Western blot
Western blot analysis of PLK1 in extracts from 293T, HeLa and A549 cell using anti-PLK1 monoclonal antibody.

Immunoprecipitation

Immunoprecipitation
Immunoprecipitation of PLK1 transfected lysate using anti-PLK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PLK1 rabbit polyclonal antibody.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged PLK1 is approximately 1 ng/ml as a capture antibody.

Requested From: United States
Date Requested: 12/9/2016

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