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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PLIN2 / ADFP / Adipophilin Antibody (aa150‑250) IHC‑plus™ LS‑B3121
Note: This antibody replaces LS-C49131
Adipophilin antibody LS-B3121 is an unconjugated rabbit polyclonal antibody to human Adipophilin (PLIN2 / ADFP). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 4 publications.
Catalog
Size
Price
LS-B3121-100
100 µl (1.23 mg/ml)
Unavailable

Popular PLIN2 / ADFP / Adipophilin Products

Antibody
Species: Human, Mouse, Rat, Bovine, Dog
Applications: IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot
Detection of murine ADFP in mouse liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Mouse
Applications: Western blot
Anti-Adipophilin antibody IHC of human liver, lipid droplets. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4850 concentration 10 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Western blot of ADFP / Adipophilin antibody LS-C146977.
Species: Human, Mouse, Rat
Applications: Western blot

Product Description

Adipophilin antibody LS-B3121 is an unconjugated rabbit polyclonal antibody to human Adipophilin (PLIN2 / ADFP). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 4 publications.
About PLIN2 / ADFP / Adipophilin
PLIN2 / ADFP / Adipophilin belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. Q99541 NM_001122 NP_001113.2

PLIN2 Antibody, Adipophilin Antibody, ADFP Antibody, ADRP Antibody, Perilipin 2 Antibody, Perilipin-2 Antibody


Specifications

Target
Human PLIN2 / ADFP / Adipophilin
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Sheep (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (2 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A synthetic peptide made to an internal portion of the human protein, within residues 150-250. [Swiss-Prot# Q99541]. Percent identity by BLAST analysis: Sheep (94%); Rat (84%).
Epitope
aa150-250
Specificity
ADFP.
Usage
Immunohistochemistry: LS-B3121 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3121 was determined to be 5 ug/ml.Recommended blocking buffer for Western blot is 5% nonfat dry milk in TBST (0.1% tween-20).
Presentation
PBS, 30% glycerol containing 0.05% sodium azide
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (4)

1
Lipid droplet breakdown requires dynamin 2 for vesiculation of autolysosomal tubules in hepatocytes. Schulze RJ, Weller SG, Schroeder B, Krueger EW, Chi S, Casey CA, McNiven MA. The Journal of cell biology. 2013 Oct;203:315-26. [Full Text Article] [PubMed:24145164] [PMC:PMC3812963]
2
Protein kinase STK25 regulates hepatic lipid partitioning and progression of liver steatosis and NASH. Cansby E, Nu[Character f1]ez-Dur[Character e1]n E, Pirazzi C, St[Character e5]hlman M, Stenfeldt E, Smith U, Bor[Character e9]n J, Mahlapuu M. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. Jan; [Full Text Article] [PubMed:25609431]
3
A novel Rab10-EHBP1-EHD2 complex essential for the autophagic engulfment of lipid droplets. Li Z, Schulze RJ, Weller SG, Krueger EW, Schott MB, Zhang X, Casey CA, Liu J, Stöckli J, James DE, McNiven MA. Science advances. 2016 2:e1601470. (WB, IF; Mouse, Human) [Full Text Article] [PubMed:28028537] [PMC:PMC5161429]
4
Clinicoplacental correlates of amniocyte vacuolization in association with gastroschisis. De Paepe ME, Mao Q, Chu S, Zhang Y, Luks FI. Placenta. 2017 57:87-93. (IHC; Human) [Full Text Article] [PubMed:28864023]
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Customer Reviews (0)


Images


Immunohistochemistry

Anti-Adipophilin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3121 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Adipophilin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3121 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of human ADFP in human liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of human ADFP in human liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-Adipophilin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3121 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Adipophilin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3121 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of human ADFP in human liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of human ADFP in human liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-Adipophilin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3121 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Adipophilin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3121 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of human ADFP in human liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of human ADFP in human liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Anti-Adipophilin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3121 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-Adipophilin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3121 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Detection of human ADFP in human liver lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Detection of human ADFP in human liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/20/2018

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