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Anti-PLIN2 / ADFP / Adipophilin Antibody (aa150-250) IHC-plus™ LS-B3121

Note: This antibody replaces LS-C49131
Catalog Size Price
LS-B3121-100 100 µl (1.39 mg/ml) Unavailable

Most Popular PLIN2 / ADFP / Adipophilin Antibodies

Anti-PLIN2 / ADFP / Adipophilin Antibody (aa1-29) LS-C3561
Guinea pig Polyclonal (IgG) to Human PLIN2 / ADFP / Adipophilin
Human, Mouse, Rat, Bovine, Dog
IHC - Paraffin, IHC - Frozen, ICC, Western blot
Unconjugated
Anti-PLIN2 / ADFP / Adipophilin Antibody (aa350-425) LS-C49130
Rabbit Polyclonal to Mouse PLIN2 / ADFP / Adipophilin
Mouse
Western blot
Unconjugated
Immunocytochemistry Image
Anti-PLIN2 / ADFP / Adipophilin Antibody (aa150-250) IHC-plus™ LS-B3121
Rabbit Polyclonal to Human PLIN2 / ADFP / Adipophilin
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PLIN2 / ADFP / Adipophilin Antibody IHC-plus™ LS-B4850
Rabbit Polyclonal to Human PLIN2 / ADFP / Adipophilin
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PLIN2 / ADFP / Adipophilin Antibody (aa5-27, clone AP125) LS-C348703
Mouse Monoclonal [clone AP125] (IgG1) to Human PLIN2 / ADFP / Adipophilin
Human, Rat, Dog
IHC - Paraffin, IHC - Frozen, ICC, Immunofluorescence, Western blot
Unconjugated

100% Guaranteed Publications (2)
Rabbit Polyclonal to Human PLIN2 / ADFP / Adipophilin
Human
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human PLIN2 / ADFP / Adipophilin
Rabbit
Human (tested or 100% immunogen sequence identity)
Sheep (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (2 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

A synthetic peptide made to an internal portion of the human protein, within residues 150-250. [Swiss-Prot# Q99541]. Percent identity by BLAST analysis: Sheep (94%); Rat (84%).
aa150-250
ADFP.
Immunohistochemistry: LS-B3121 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3121 was determined to be 5 ug/ml.Recommended blocking buffer for Western blot is 5% nonfat dry milk in TBST (0.1% tween-20).

Packaging

PBS, 30% glycerol containing 0.05% sodium azide
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
For research use only.

Publications (2)

1
Lipid droplet breakdown requires dynamin 2 for vesiculation of autolysosomal tubules in hepatocytes. Schulze RJ, Weller SG, Schroeder B, Krueger EW, Chi S, Casey CA, McNiven MA. The Journal of cell biology. 2013 Oct;203:315-26. [Full Text Article] [PubMed:24145164] [PMC:PMC3812963]
2
Protein kinase STK25 regulates hepatic lipid partitioning and progression of liver steatosis and NASH. Cansby E, Nu[Character f1]ez-Dur[Character e1]n E, Pirazzi C, St[Character e5]hlman M, Stenfeldt E, Smith U, Bor[Character e9]n J, Mahlapuu M. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. Jan; [Full Text Article] [PubMed:25609431]

About PLIN2 / ADFP / Adipophilin

Q99541 NM_001122 NP_001113.2

PLIN2 Antibody, Adipophilin Antibody, ADFP Antibody, ADRP Antibody, Perilipin 2 Antibody, Perilipin-2 Antibody

PLIN2 / ADFP / Adipophilin belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue.

Immunohistochemistry

Immunohistochemistry
Anti-Adipophilin antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3121 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Detection of human ADFP in human liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 8/21/2017

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