Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Mouse, Rat, Bovine, Dog
(tested or 100% immunogen sequence identity)
IHC - Paraffin
IHC - Frozen
Western blot (1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Synthetic peptide (N-terminal aa 1-29 of human and murine adipophilin).
Recognizes Adipophilin at ~52kD Reactive with cell lines Caco, PLC, HaCat, SV80, RD 125, Huvec. Does not react with glioma. Species cross-reactivity: Human, mouse, rat, canine, bovine.
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Western Blot: 1:2000. Immunohistochemistry (Frozen, Paraffin embedded tissue after microwave treatment): 1:100; Incubate for 1hr at RT or overnight at 4?C. Positive control: Glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver (adipocytes are negative), lipid storing CD68-positive macrophages.
0.09% Sodium Azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
PLIN2 / ADFP / Adipophilin belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue.