Adipophilin antibody LS-C3561 is an unconjugated guinea pig polyclonal antibody to Adipophilin (PLIN2 / ADFP) from human, mouse, rat and other species. Validated for ICC, IHC and WB.
Human PLIN2 / ADFP / Adipophilin
Human, Mouse, Rat, Bovine, Dog
(tested or 100% immunogen sequence identity)
- IHC - Paraffin
- IHC - Frozen
- Western blot (1:2000)
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Synthetic peptide (N-terminal aa 1-29 of human and murine adipophilin).
Recognizes Adipophilin at ~52kD Reactive with cell lines Caco, PLC, HaCat, SV80, RD 125, Huvec. Does not react with glioma. Species cross-reactivity: Human, mouse, rat, canine, bovine.
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Western Blot: 1:2000. Immunohistochemistry (Frozen, Paraffin embedded tissue after microwave treatment): 1:100; Incubate for 1hr at RT or overnight at 4?C. Positive control: Glandular cells of lactating mammary gland (ductal cells are negative), zona fasciculata of the adrenal gland, Sertoli cells of the testis, fat-accumulating hepatocytes of alcoholic cirrhotic fatty liver (adipocytes are negative), lipid storing CD68-positive macrophages.
0.09% Sodium Azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.
About PLIN2 / ADFP / Adipophilin