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Anti-PLEKHH2 Antibody (aa1043-1092) IHC-plus™ LS-B3567

Note: This antibody replaces LS-C82718

Ordering

Wt. Vol. Conc. Price
50 µg - - $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PLEKHH2 Antibodies

Anti-PLEKHH2 Antibody (aa1043-1092) IHC-plus™ LS-B3567
Rabbit Polyclonal (IgG) to Human PLEKHH2
Human, Monkey, Mouse, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PLEKHH2 Antibody (aa1049-1079, Biotin) LS-C239117
Rabbit Polyclonal (IgG) to Human PLEKHH2
Human
IHC, Western blot, Flow Cytometry, ELISA
Biotin Conjugated
Anti-PLEKHH2 Antibody (aa1049-1079, Azide-free, HRP) LS-C248488
Rabbit Polyclonal (IgG) to Human PLEKHH2
Human
IHC, Western blot, Flow Cytometry, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human PLEKHH2
Human, Monkey, Mouse, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human PLEKHH2
Rabbit
Human, Monkey, Mouse, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Rat (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with HRP, Biotin, FITC.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

PLEKHH2 antibody was raised against synthetic peptide located between aa1043-1092 of human PLEKHH2 (Q8IVE3, NP_742066). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Zebra finch (100%); Rat, Opossum (92%); Turkey, Chicken (86%); Xenopus, Drosophila (84%).
LS-E19208 - Lyophilized - 100 µg - $145.00
aa1043-1092
Human PLEKHH2
Immunohistochemistry: LS-B3567 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3567 was determined to be 10 ug/ml. ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled Water.
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About PLEKHH2

Q8IVE3 Q8IVE3

PLEKHH2 Antibody, KIAA2028 Antibody, PLEKHH1L Antibody

In the kidney glomerulus may play a role in linking podocyte foot processes to the glomerular basement membrane. May be involved in stabilization of F-actin by attenuating its depolymerization. Can recruit TGFB1I1 from focal adhesions to podocyte lamellipodia.

Immunohistochemistry

Immunohistochemistry
Anti-PLEKHH2 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3567 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
Anti-PLEKHH2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3567 concentration 10 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Jurkat cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 6-18%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/4/2016

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