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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PLDN / Pallidin Antibody (clone 1H9) LS‑C173735 Best Seller
Pallidin antibody LS-C173735 is an unconjugated mouse monoclonal antibody to Pallidin (PLDN) from human and mouse. Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C173735-100
100 µl (0.6 mg/ml)
Unavailable

Popular PLDN / Pallidin Products

IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PLDN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Rabbit antibody to Pallidin (50-100). IHC-P on paraffin sections of human stomach. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered through a 0.2 micron filter. Detection was done using Novolink HRP polymer from Leica following manufacturers instructions. Primary antibody: dilution 1:1000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Species: Human, Mouse
Applications: IHC, Western blot
Immunohistochemistry of paraffin-embedded human pancreatic cancer at dilution of 1:100
Species: Human
Applications: IHC, Western blot, ELISA
Immunohistochemistry of paraffin-embedded human pancreatic cancer at dilution of 1:100
Species: Human
Applications: IHC, Western blot, Immunoprecipitation, ELISA

Product Description

Pallidin antibody LS-C173735 is an unconjugated mouse monoclonal antibody to Pallidin (PLDN) from human and mouse. Validated for Flow, IHC and WB.
About PLDN / Pallidin
Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Q9UL45 NM_012388 NP_036520.1

BLOC1S6 Antibody, HPS9 Antibody, Pallid protein homolog Antibody, Pallidin Antibody, Pallidin homolog (mouse) Antibody, PLDN Antibody, Syntaxin 13 binding protein 1 Antibody, Pa Antibody, PALLID Antibody


Specifications

Target
Human PLDN / Pallidin
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [1H9]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:150)
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
PLDN / Pallidin antibody was raised against full length human recombinant protein of human PLDN(NP_036520) produced in HEK293T cell.
Specificity
Human PLDN / Pallidin
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

IHC of paraffin-embedded Human tonsil using anti-PLDN mouse monoclonal antibody.
IHC of paraffin-embedded Human tonsil using anti-PLDN mouse monoclonal antibody.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-PLDN monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-PLDN monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLDN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLDN.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLDN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLDN.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PLDN antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PLDN antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Human tonsil using anti-PLDN mouse monoclonal antibody.
IHC of paraffin-embedded Human tonsil using anti-PLDN mouse monoclonal antibody.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-PLDN monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-PLDN monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLDN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLDN.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLDN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLDN.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PLDN antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PLDN antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Human tonsil using anti-PLDN mouse monoclonal antibody.
IHC of paraffin-embedded Human tonsil using anti-PLDN mouse monoclonal antibody.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-PLDN monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-PLDN monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLDN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLDN.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLDN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLDN.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PLDN antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PLDN antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Human tonsil using anti-PLDN mouse monoclonal antibody.
IHC of paraffin-embedded Human tonsil using anti-PLDN mouse monoclonal antibody.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-PLDN monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-PLDN monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLDN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLDN.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLDN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLDN.

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PLDN antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PLDN antibody, and then analyzed by flow cytometry.


Requested From: 
Date Requested: 6/19/2018

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