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Anti-PLCG2 / PLC Gamma 2 Antibody (aa400-600, clone 346404) LS-C150115

Ordering

Wt. Vol. Conc. Price
100 µg - 1 mg/ml $315
Inquire for larger quantities

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Mouse Monoclonal [clone 346404] (IgG1) to Human PLCG2 / PLC Gamma 2
Human, Mouse
ICC, Western blot
Unconjugated

Details

Human PLCG2 / PLC Gamma 2
Mouse
Human, Mouse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [346404]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • ICC (8 - 25 µg/ml)
  • Western blot (1 µg/ml)

Specificity and Use

PLCG2 / PLC Gamma 2 antibody was raised against e. coli-derived recombinant human PLCgamma2 Pro400-Tyr600 Accession # P16885.
aa400-600
Detects human and mouse PLC gamma 2.

Packaging

Lyophilized from 0.2 um sterile-filtered solution in PBS, 5% trehalose
Sterile PBS, 0.02% sodium azide
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About PLCG2 / PLC Gamma 2

P16885 NM_002661 NP_002652.2

PLCG2 Antibody, FCAS3 Antibody, Phospholipase C-gamma-2 Antibody, PLCG 2 Antibody, PLC-IV Antibody, APLAID Antibody, Phospholipase C-IV Antibody, PLC-gamma-2 Antibody

PLCG2 / PLC Gamma 2 is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane.

Immunocytochemistry

Immunocytochemistry
PLC- gamma 2 in Human PBMCs. PLC- gamma 2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human/Mouse PLC- gamma 2 Monoclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow) and counterstained with DAPI (blue).

Western blot

Western blot
Detection of Human/Mouse PLC- gamma 2 by Western Blot. Western blot shows lysates of A20 mouse B cell lymphoma cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 ug/ml of Human/Mouse PLC- gamma 2 Monoclonal Antibody followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for PLC- gamma 2 at approximately 145 kD (as indicated). This experiment was conducted under reducing conditions.

Requested From: United States
Date Requested: 12/7/2016

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