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Anti-PLCG1 Antibody (phospho-Tyr783) LS-C9600

Catalog	Size	Price
LS-C9600-100	100 µl (0.02 mg/ml)	Unavailable

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Western blot analysis using PLCG1 mAb against human PLCG1 (AA: 39-181) recombinant protein. (Expected MW is 43 kDa)

Antibody:	Rabbit Polyclonal (IgG) to Human PLCG1
Reactivity:	Human, Mouse
Application:	Immunofluorescence, Western blot, Flow Cytometry, ELISA
Format:	Unconjugated

Details

Target:	Human PLCG1
Host:	Rabbit
Reactivity:	Human, Mouse (tested or 100% immunogen sequence identity)
Clonality:	IgG Polyclonal
Conjugations:	Unconjugated
Purification:	Protein A purified
Modifications:	Unmodified

Applications

Approved:

Immunofluorescence
Western blot (1:1000)
Flow Cytometry (1:25)
ELISA

Specificity and Use

Immunogen:	PLCG1 antibody was raised against synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr783 of human PLC gamma 1.
Epitope:	pTyr783
Specificity:	Recognizes human PLC gamma 1 only when activated by phosphorylation at Tyr783. It does not cross-react with phosphorylated PLC gamma 2 or other PLCs. Species cross-reactivity: Mouse. Species sequence Homology: Rat - 100%.
Usage:	Suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry. Western Blot: 1:1000 incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Flow Cytometry: 1:25.

Packaging

Presentation:	10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol. No preservative added.
Storage:	Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions:	For research use only.

About PLCG1

Uniprot: P19174 NCBI: NM_002660 NP_002651.2

Synonyms: PLCG1 Antibody, Phospholipase C, gamma 1 Antibody, Phospholipase C-148 Antibody, Phosphoinositidase C Antibody, Phospholipase C-II Antibody, Inositoltrisphosphohydrolase Antibody, PLC-gamma-1 Antibody, PLC1 Antibody, PLC-148 Antibody, PLC-II Antibody, PLC148 Antibody, NCKAP3 Antibody, Phospholipase C-gamma-1 Antibody, PLCgamma1 Antibody

Description: PLCG1 catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras.

(More About PLCG1)

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