(tested or 100% immunogen sequence identity)
Protein A purified
- Western blot (1:1000)
- Flow Cytometry (1:25)
PLCG1 antibody was raised against synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr783 of human PLC gamma 1.
Recognizes human PLC gamma 1 only when activated by phosphorylation at Tyr783. It does not cross-react with phosphorylated PLC gamma 2 or other PLCs. Species cross-reactivity: Mouse. Species sequence Homology: Rat - 100%.
Suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry. Western Blot: 1:1000 incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Flow Cytometry: 1:25.
10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol. No preservative added.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.