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PLCG1 antibody LS-C9600 is an unconjugated rabbit polyclonal antibody to PLCG1 from human and mouse. Validated for ELISA, Flow, IF and WB.
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Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse, Rat, Bovine
Applications:
IHC, IHC - Paraffin, Western blot
Product Description
PLCG1 antibody LS-C9600 is an unconjugated rabbit polyclonal antibody to PLCG1 from human and mouse. Validated for ELISA, Flow, IF and WB.
Specifications
Target
Human PLCG1
Synonyms
PLCG1, Phospholipase C, gamma 1, Phospholipase C-148, Phosphoinositidase C, Phospholipase C-II, Inositoltrisphosphohydrolase, PLC-gamma-1, PLC1, PLC-148, PLC-II, PLC148, NCKAP3, Phospholipase C-gamma-1, PLCgamma1
Host
Rabbit
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Applications
- Immunofluorescence
- Western blot (1:1000)
- Flow Cytometry (1:25)
- ELISA
Immunogen
PLCG1 antibody was raised against synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr783 of human PLC gamma 1.
Epitope
pTyr783
Specificity
Recognizes human PLC gamma 1 only when activated by phosphorylation at Tyr783. It does not cross-react with phosphorylated PLC gamma 2 or other PLCs. Species cross-reactivity: Mouse. Species sequence Homology: Rat - 100%.
Usage
Suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry. Western Blot: 1:1000 incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Flow Cytometry: 1:25.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About PLCG1
PLCG1 catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras.
P19174
NM_002660
NP_002651.2
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Requested From: United States
Date Requested: 10/19/2018
Date Requested: 10/19/2018
