LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
PLCG1 Antibody (phospho‑Tyr771) LS‑C9602
PLCG1 antibody LS-C9602 is an unconjugated rabbit polyclonal antibody to PLCG1 from human, mouse, rat and other species. Validated for WB.
Antibody
Catalog
Size
Price
LS-C9602-200
200 µg
$590

Popular PLCG1 Products

Anti-PLCG1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7825 dilution 1:200.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Anti-PLCG1 antibody IHC of human colon, lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2311 dilution 1:25.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PLCG1 (Phospho-Tyr783) Antibody. The picture on the right is blocked with the phospho peptide.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemical analysis of PLC gamma 1 (pY783) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat, Bovine
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Human, Mouse, Rat
Applications: Western blot

Product Description

PLCG1 antibody LS-C9602 is an unconjugated rabbit polyclonal antibody to PLCG1 from human, mouse, rat and other species. Validated for WB.
About PLCG1
PLCG1 catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. P19174 NM_002660 NP_002651.2

PLCG1 Antibody, Phospholipase C, gamma 1 Antibody, Phospholipase C-148 Antibody, Phosphoinositidase C Antibody, Phospholipase C-II Antibody, Inositoltrisphosphohydrolase Antibody, PLC-gamma-1 Antibody, PLC1 Antibody, PLC-148 Antibody, PLC-II Antibody, PLC148 Antibody, NCKAP3 Antibody, Phospholipase C-gamma-1 Antibody, PLCgamma1 Antibody


Specifications

Target
Human PLCG1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
PLCG1 antibody was raised against kLH-conjugated, synthetic peptide surrounding phospho-Tyr771 of human PLCg-1.
Epitope
pTyr771
Specificity
Recognizes PLCg-1, Mr ~135kD, phosphorylated at Tyr771. Species cross-reactivity: Human. Expected cross-reactivity with bovine, mouse and rat, based on sequence homology.
Usage
Suitable for use in Western Blot. Western Blot Analysis: 1-2 ug/ml of this lot detected phospho-PLCg-1 in RIPA lysates from EGF-stimulated A431 cells.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

Publications (0)


Customer Reviews (0)


Requested From: United States
Date Requested: 9/24/2018

Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy