PLCG1 antibody LS-C9591 is an unconjugated mouse monoclonal antibody to PLCG1 from human, mouse, rat and other species. Validated for ELISA, ICC, IP and WB.
Bovine, Human, Mouse, Rat, Rabbit
(tested or 100% immunogen sequence identity)
Protein G purified
- ICC (5 - 10 µg/ml)
- Western blot (0.5 - 2 µg/ml)
PLCG1 antibody was raised against purified soluble phospholipase C (PLC) g-1 from bovine brain.
Recognizes bovine PLC g-1 at 135kD. Reacts with some non-PLC g-1 SH-3 containing proteins, including nck (47kD). Species cross-reactivity: rabbit, rat, mouse and human.
Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunocytochemistry. Western Blot: 0.5-2 ug/ml detects PLCg1 in human A431 carcinoma cells as well as rabbit and bovine brain cytosol preparations. Immunoprecipitation: 4 ug Immunoprecipitates PLCg1 from non-stimulated A431 cell lysates and from rabbit brain cytosol preparation. Immunocytochemistry: 5-10 ug/ml detects PLCg1 on ethanol-acetic acid (95:5) fixed A431 cells.
0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, 0.05% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.