Bovine, Human, Mouse, Rat, Rabbit
(tested or 100% immunogen sequence identity)
Protein G purified
- ICC (5 - 10 µg/ml)
- Western blot (0.5 - 2 µg/ml)
PLCG1 antibody was raised against purified soluble phospholipase C (PLC) g-1 from bovine brain.
Recognizes bovine PLC g-1 at 135kD. Reacts with some non-PLC g-1 SH-3 containing proteins, including nck (47kD). Species cross-reactivity: rabbit, rat, mouse and human.
Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunocytochemistry. Western Blot: 0.5-2 ug/ml detects PLCg1 in human A431 carcinoma cells as well as rabbit and bovine brain cytosol preparations. Immunoprecipitation: 4 ug Immunoprecipitates PLCg1 from non-stimulated A431 cell lysates and from rabbit brain cytosol preparation. Immunocytochemistry: 5-10 ug/ml detects PLCg1 on ethanol-acetic acid (95:5) fixed A431 cells.
0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, 0.05% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.