LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
PLCG1 Antibody LS‑C9591
PLCG1 antibody LS-C9591 is an unconjugated mouse monoclonal antibody to PLCG1 from human, mouse, rat and other species. Validated for ELISA, ICC, IP and WB.
Antibody
Catalog
Size
Price
LS-C9591-50
50 µg (1.25 mg/ml)
$605

Popular PLCG1 Products

Anti-PLCG1 antibody IHC of human colon, lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2311 dilution 1:25.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Antibody
Species: Human, Mouse, Rat
Applications: Western blot
Anti-PLCG1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7825 dilution 1:200.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PLCG1 (Phospho-Tyr783) Antibody. The picture on the right is blocked with the phospho peptide.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Immunohistochemical analysis of PLC gamma 1 (pY783) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat, Bovine
Applications: IHC, IHC - Paraffin, Western blot

Product Description

PLCG1 antibody LS-C9591 is an unconjugated mouse monoclonal antibody to PLCG1 from human, mouse, rat and other species. Validated for ELISA, ICC, IP and WB.
About PLCG1
PLCG1 catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. P19174 NM_002660 NP_002651.2

PLCG1 Antibody, Phospholipase C, gamma 1 Antibody, Phospholipase C-148 Antibody, Phosphoinositidase C Antibody, Phospholipase C-II Antibody, Inositoltrisphosphohydrolase Antibody, PLC-gamma-1 Antibody, PLC1 Antibody, PLC-148 Antibody, PLC-II Antibody, PLC148 Antibody, NCKAP3 Antibody, Phospholipase C-gamma-1 Antibody, PLCgamma1 Antibody


Specifications

Target
Bovine PLCG1
Host
Mouse
Reactivity
Bovine, Human, Mouse, Rat, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • ICC (5 - 10 µg/ml)
  • Western blot (0.5 - 2 µg/ml)
  • Immunoprecipitation
  • ELISA
Immunogen
PLCG1 antibody was raised against purified soluble phospholipase C (PLC) g-1 from bovine brain.
Specificity
Recognizes bovine PLC g-1 at 135kD. Reacts with some non-PLC g-1 SH-3 containing proteins, including nck (47kD). Species cross-reactivity: rabbit, rat, mouse and human.
Usage
Suitable for use in ELISA, Immunoprecipitation, Western Blot and Immunocytochemistry. Western Blot: 0.5-2 ug/ml detects PLCg1 in human A431 carcinoma cells as well as rabbit and bovine brain cytosol preparations. Immunoprecipitation: 4 ug Immunoprecipitates PLCg1 from non-stimulated A431 cell lysates and from rabbit brain cytosol preparation. Immunocytochemistry: 5-10 ug/ml detects PLCg1 on ethanol-acetic acid (95:5) fixed A431 cells.
Presentation
0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, 0.05% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Requested From: United States
Date Requested: 8/14/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number