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Anti-PLA2G5 Antibody (clone MCL-3G1) IHC-plus™ LS-B1613

Note: This antibody replaces LS-C11702

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PLA2G5 Antibodies

Anti-PLA2G5 Antibody (clone MCL-3G1) IHC-plus™ LS-B1613
Mouse Monoclonal [clone MCL-3G1] (IgG1) to Human PLA2G5
Human
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-PLA2G5 Antibody (aa103-132) LS-C163763
Rabbit Polyclonal to Human PLA2G5
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PLA2G5 Antibody LS-C317545
Rabbit Polyclonal (IgG) to Mouse PLA2G5
Mouse
ELISA
Unconjugated

100% Guaranteed 100% Guaranteed Publications Publications (1)
Mouse Monoclonal [clone MCL-3G1] (IgG1) to Human PLA2G5
Human
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated

Details

Human PLA2G5
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [MCL-3G1]
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot
  • Immunoprecipitation

Specificity and Use

PLA2G5 antibody was raised against recombinant human PLA2G5.
Purified recombinant human sPLA2 (type V)
Immunohistochemistry: LS-B1613 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1613 was determined to be 10 ug/ml.

Packaging

PBS, pH 7.4.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

Publications (1)

1
Biallelic mutations in PLA2G5, encoding group V phospholipase A2, cause benign fleck retina. Sergouniotis PI, Davidson AE, Mackay DS, Lenassi E, Li Z, Robson AG, Yang X, Kam JH, Isaacs TW, Holder GE, Jeffery G, Beck JA, Moore AT, Plagnol V, Webster AR. American journal of human genetics. 2011 Dec;89:782-91. [Full Text Article] [PubMed:22137173] [PMC:PMC3234375]

About PLA2G5

P39877 NM_000929 NP_000920.1

PLA2G5 Antibody, GV-PLA2 Antibody, HVPLA(2) Antibody, FRFB Antibody, Phospholipase A2, group V Antibody, PLA2-10 Antibody, Group V phospholipase A2 Antibody

This gene is a member of the secretory phospholipase A2 family. It is located in a tightly-linked cluster of secretory phospholipase A2 genes on chromosome 1. The encoded enzyme catalyzes the hydrolysis of membrane phospholipids to generate lysophospholipids and free fatty acids including arachidonic acid. It preferentially hydrolyzes linoleoyl-containing phosphatidylcholine substrates. Secretion of this enzyme is thought to induce inflammatory responses in neighboring cells.

Immunohistochemistry

Immunohistochemistry
Anti-PLA2G5 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1613 concentration 10 ug/ml.

Western blot

Western blot
Western blot of PLA2G5 antibody LS-B1613.

Requested From: United States
Date Requested: 12/9/2016

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