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Catalog Number Size Price
LS-B5952-50 50 µg (0.5 mg/ml) $485 
PLA2G4A Antibody - Anti-PLA2G4A antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
PLA2G4A Antibody - PLA2G4A antibody immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
PLA2G4A Antibody - PLA2G4A antibody (1µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
PLA2G4A Antibody - PLA2G4A antibody (1µg/ml) staining of A549 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
PLA2G4A Antibody - PLA2G4A antibody (1 ug/ml) staining of HeLa lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
PLA2G4A Antibody - PLA2G4A antibody flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
PLA2G4A Antibody - Anti-PLA2G4A antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
PLA2G4A Antibody - PLA2G4A antibody immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
PLA2G4A Antibody - PLA2G4A antibody (1µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
PLA2G4A Antibody - PLA2G4A antibody (1µg/ml) staining of A549 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
PLA2G4A Antibody - PLA2G4A antibody (1 ug/ml) staining of HeLa lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
PLA2G4A Antibody - PLA2G4A antibody flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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IHC‑plus™ Polyclonal Goat anti‑Human PLA2G4A Antibody (aa153‑166, IHC, IF, WB) LS‑B5952

IHC‑plus™ Polyclonal Goat anti‑Human PLA2G4A Antibody (aa153‑166, IHC, IF, WB) LS‑B5952

Note: This antibody replaces LS-C113010
Antibody:
PLA2G4A Goat anti-Human Polyclonal (aa153-166) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B5952-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
PLA2G4A Goat anti-Human Polyclonal (aa153-166) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PLA2G4A antibody LS-B5952 is an unconjugated goat polyclonal antibody to human PLA2G4A (aa153-166). Validated for IF, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human PLA2G4A
Synonyms
PLA2G4A | CPLA2 | CPLA2-alpha | Cytosolic phospholipase A2 | Lysophospholipase | Phospholipase A2 group IVA | PLA2G4
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-QEKTFRQQRKEHIR, from the internal region of the protein sequence according to NP_077734.1.
Epitope
aa153-166
Specificity
Human PLA2G4A / Phospholipase A2.
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Immunofluorescence
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 90kDa band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 85.2kDa according to NP_077734.1). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PLA2G4A
P47712 NM_024420 AAA60105.1

Validation

PLA2G4A Antibody - Anti-PLA2G4A antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-PLA2G4A antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-PLA2G4A antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

LSBio Ratings

IHC-plus™ PLA2G4A Antibody (aa153-166) for IHC, IF/Immunofluorescence, WB/Western LS-B5952 has an LSBio Rating of
Laboratory Validation Score (4)

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Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/25/2024