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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PLA2G1B Antibody (aa134‑148) LS‑C55295 Best Seller
PLA2G1B antibody LS-C55295 is an unconjugated goat polyclonal antibody to human PLA2G1B. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C55295-100
100 µg (0.5 mg/ml)
Unavailable

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Applications: Western blot, Flow Cytometry, ELISA

Product Description

PLA2G1B antibody LS-C55295 is an unconjugated goat polyclonal antibody to human PLA2G1B. Validated for Peptide-ELISA and WB.
About PLA2G1B
PLA2G1B is a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. The encoded calcium-dependent enzyme catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids. AA is subsequently converted by downstream metabolic enzymes to several bioactive lipophilic compounds (eicosanoids), including prostaglandins (PGs) and leukotrienes (LTs). P04054 NM_000928 NP_000919.1

PLA2G1B Antibody, Group IB phospholipase A2 Antibody, Phospholipase A2 Antibody, PLA2A Antibody, PPLA2 Antibody, PLA2 Antibody


Specifications

Target
Human PLA2G1B
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1:64000) & (0.01 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Failed Applications
  • IHC - Paraffin
Immunogen
PLA2G1B antibody was raised against synthetic peptide NKAHKNLDTKKYCQS from the C-terminus of human PLA2G1B (NP_000919.1). Percent identity by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey (87%); Marmoset, Panda, Dog, Pig (80%).
Blocking Peptide
LS-E26464 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C55295. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa134-148
Specificity
Human PLA2G1B.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Western blot

HEK293 overexpressing PLA2G1B (RC216089) and probed with (mock transfection in first lane).
HEK293 overexpressing PLA2G1B (RC216089) and probed with (mock transfection in first lane).

Western blot

Antibody (0.01 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.01 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

HEK293 overexpressing PLA2G1B (RC216089) and probed with (mock transfection in first lane).
HEK293 overexpressing PLA2G1B (RC216089) and probed with (mock transfection in first lane).

Western blot

Antibody (0.01 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.01 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

HEK293 overexpressing PLA2G1B (RC216089) and probed with (mock transfection in first lane).
HEK293 overexpressing PLA2G1B (RC216089) and probed with (mock transfection in first lane).

Western blot

Antibody (0.01 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.01 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

HEK293 overexpressing PLA2G1B (RC216089) and probed with (mock transfection in first lane).
HEK293 overexpressing PLA2G1B (RC216089) and probed with (mock transfection in first lane).

Western blot

Antibody (0.01 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.01 ug/ml) staining of Human Pancreas lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.


Requested From: 
Date Requested: 6/24/2018

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