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Anti-PKP3 / Plakophilin 3 Antibody LS-C26079

Ordering

Wt. Vol. Conc. Price
100 µg - - Unavailable

Most Popular PKP3 / Plakophilin 3 Antibodies

Anti-PKP3 / Plakophilin 3 Antibody (aa569-589) LS-C26078
Mouse Monoclonal (IgG2b) to Human PKP3 / Plakophilin 3
Human
IHC - Paraffin, IHC - Frozen, ICC, Western blot, Immunoprecipitation
Unconjugated
Anti-PKP3 / Plakophilin 3 Antibody (clone 12B11F8) LS-C146790
Mouse Monoclonal [clone 12B11F8] (IgG2a) to Human PKP3 / Plakophilin 3
Human, Mouse, Dog, Pig, Xenopus
IHC - Frozen, ICC, Western blot, Immunoprecipitation
Unconjugated
Anti-PKP3 / Plakophilin 3 Antibody (clone 23E3/4) IHC-plus™ LS-B7190
Mouse Monoclonal [clone 23E3/4] (IgG2b) to Human PKP3 / Plakophilin 3
Human, Mouse, Dog
IHC - Paraffin, IHC - Frozen, ICC, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG2b) to Human PKP3 / Plakophilin 3
Human, Mouse, Dog
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA (applications tested for the base form of this product only)
Unconjugated

Details

Human PKP3 / Plakophilin 3
Mouse
Human, Mouse, Dog (tested or 100% immunogen sequence identity)
IgG2b Monoclonal
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, MaxLight 405, MaxLight 490, MaxLight 550, MaxLight 650, MaxLight 750, PE.
Protein A purified
Unmodified

Applications

  • IHC - Paraffin
  • Immunofluorescence (1 - 3 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Immunoprecipitation
  • ELISA (0.1 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

PKP3 / Plakophilin 3 antibody was raised against synthetic peptide from Armadillo domain of human Plakophilin-3.
Reacts with the 87kD human Plakophilin-3. There is no cross reactivity with human Plakophilin-1 and Plakophilin-2 proteins. Reactivity is confirmed with human A431 cells, and HEK293 cells transfected with plasmid expressing human Plakophilin-3. Based on sequence homology, this antibody is expected to react with mouse and canine.
Suitable for use in ELISA, Immunoprecipitation (native), Immunohistochemistry (formalin fixed paraffin embedded), Immunofluorescence and Western Blot. ELISA: 0.1-1 ug/ml. Immunoprecipitation: 10 ug/IP reaction. Immunohistochemistry: 10 ug/ml. Immunofluorescence: 1-3 ug/ml. Western Blot: 1-3 ug/ml. Note: Immunofluorescent assays were performed using methanol-fixed HCT8/E8 and paraformaldehyde-fixed HaCat cells. For optimal IF results, methanol and paraformaldehyde- fixed cells should be permeabilized with 0.2% Triton X-100 for 5 and 15 minutes, respectively, prior to incubation with primary antibody.

Packaging

PBS, pH 7.4, 0.1% sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About PKP3 / Plakophilin 3

Q9Y446 NM_007183 NP_009114.1

PKP3 Antibody, Plakophilin 3 Antibody, Plakophilin-3 Antibody, Plakophilin 3b Antibody

PKP3 / Plakophilin 3 is a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This protein may act in cellular desmosome-dependent adhesion and signaling pathways. Two transcript variants encoding different isoforms have been found for this gene.

Requested From: 
Date Requested: 3/26/2017

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