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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PKP2 / Plakophilin 2 Antibody (aa125‑139) IHC‑plus™ LS‑B9231
Note: This antibody replaces LS-C139767
Plakophilin 2 antibody LS-B9231 is an unconjugated goat polyclonal antibody to Plakophilin 2 (PKP2) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B9231-50
50 µg (0.5 mg/ml)
Unavailable

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Product Description

Plakophilin 2 antibody LS-B9231 is an unconjugated goat polyclonal antibody to Plakophilin 2 (PKP2) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
About PKP2 / Plakophilin 2
PKP2 / Plakophilin 2 is a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. This gene product may regulate the signaling activity of beta-catenin. Two alternately spliced transcripts encoding two protein isoforms have been identified. Q99959 NM_004572 NP_004563.2

PKP2 Antibody, ARVD9 Antibody, Plakophilin 2 Antibody, Plakophilin-2 Antibody


Specifications

Target
Human PKP2 / Plakophilin 2
Host
Goat
Reactivity
Human, Mouse, Rat, Dog, Hamster, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bat, Bovine, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide C-RGTAQYSSQKSVEER from an internal region of human PKP2 / Plakophilin 2 (NP_001005242.2; NP_004563.2). Percent identity by BLAST analysis: Human, Gorilla, Hamster, Dog, Rabbit (100%); Marmoset, Panda, Bovine, Bat, Horse (93%); Mouse (87%); Elephant, Opossum (80%).
Blocking Peptide
LS-E27654 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B9231. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa125-139
Specificity
Human PKP2 / Plakophilin 2. This antibody is expected to recognize both reported isoforms (NP_001005242.2; NP_004563.2).
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 100kD band observed in Mouse and Rat Heart lysates (calculated MW of 88.1kD according to Mouse NP_080439.1 and 87.8kD according to Rat NP_001093969.1). Recommended concentration: 0.3-1 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-PKP2 / Plakophilin 2 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PKP2 / Plakophilin 2 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

PKP2 antibody (0.3 ug/ml) staining of Mouse Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
PKP2 antibody (0.3 ug/ml) staining of Mouse Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-PKP2 / Plakophilin 2 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PKP2 / Plakophilin 2 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

PKP2 antibody (0.3 ug/ml) staining of Mouse Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
PKP2 antibody (0.3 ug/ml) staining of Mouse Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-PKP2 / Plakophilin 2 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PKP2 / Plakophilin 2 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

PKP2 antibody (0.3 ug/ml) staining of Mouse Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
PKP2 antibody (0.3 ug/ml) staining of Mouse Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-PKP2 / Plakophilin 2 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-PKP2 / Plakophilin 2 antibody IHC staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

PKP2 antibody (0.3 ug/ml) staining of Mouse Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
PKP2 antibody (0.3 ug/ml) staining of Mouse Heart lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.


Requested From: 
Date Requested: 6/23/2018

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