Human, Monkey, Mouse, Rat, Dog, Hamster
(tested or 100% immunogen sequence identity)
Bovine, Pig (at least 90% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- Western blot (1 - 2 µg/ml)
- (applications tested for the base form of this product only)
PKN1 antibody was raised against kLH-conjugated, synthetic peptide corresponding to amino acids 918-930 (C-PRDARPLTAAEQA) of human Protein Kinase C Related Protein Kinase 1 (PRK1), with an N-terminal cysteine added for conjugation purposes. The immunizing sequence is 100% identical in mouse and rat. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Dog (100%); Elephant, Panda, Bovine, Pig (92%); Bat (85%).
Recognizes PRK1, Mr ~130kD. Species cross-reactivity: Human. Predicted to cross-react with mouse and rat based on sequence homology.
Suitable for use in Western Blot. Western Blot: 1-2 ug/ml detects PRK1 in RIPA lysates from Jurkat, HeLa, HEK293 and A549 cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PRK1 (1 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.