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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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PKN1 Antibody (aa918‑930) LS‑C17471
Antibody
PKN1 antibody LS-C17471 is an unconjugated rabbit polyclonal antibody to PKN1 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C17471-200
200 µg
Unavailable

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Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Flow Cytometry, ELISA

Product Description

PKN1 antibody LS-C17471 is an unconjugated rabbit polyclonal antibody to PKN1 from human, mouse, rat and other species. Validated for WB.
About PKN1
PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Q16512 NM_002741 NP_002732.3

PKN1 Antibody, PKN Antibody, Protein kinase C-like PKN Antibody, Protein kinase N1 Antibody, PRK1 Antibody, PRKCL1 Antibody, Serine-threonine kinase N Antibody, Pknalpha Antibody, Protease-activated kinase 1 Antibody, Protein kinase PKN-alpha Antibody, DBK Antibody, PAK-1 Antibody, PKN-ALPHA Antibody, Protein kinase C-like 1 Antibody


Specifications

Target
Human PKN1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Dog, Hamster (tested or 100% immunogen sequence identity)
Predicted
Bovine, Pig (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • Western blot (1 - 2 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
PKN1 antibody was raised against kLH-conjugated, synthetic peptide corresponding to amino acids 918-930 (C-PRDARPLTAAEQA) of human Protein Kinase C Related Protein Kinase 1 (PRK1), with an N-terminal cysteine added for conjugation purposes. The immunizing sequence is 100% identical in mouse and rat. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Hamster, Dog (100%); Elephant, Panda, Bovine, Pig (92%); Bat (85%).
Epitope
aa918-930
Specificity
Recognizes PRK1, Mr ~130kD. Species cross-reactivity: Human. Predicted to cross-react with mouse and rat based on sequence homology.
Usage
Suitable for use in Western Blot. Western Blot: 1-2 ug/ml detects PRK1 in RIPA lysates from Jurkat, HeLa, HEK293 and A549 cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-PRK1 (1 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Presentation
0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/25/2018

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