Rabbit Pyruvate Kinase

Goat

Rabbit (tested or 100% immunogen sequence identity)

IgG Polyclonal

Biotin

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Lyophilized

Pyruvate Kinase [Rabbit Muscle].

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyruvate Kinase [Rabbit Muscle]. Anti-Pyruvate Kinase has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross reactivity against Pyruvate Kinase from other sources may occur but have not been specifically determined.

• Western blot (1:200 - 1:1000)
• ELISA (1:1000 - 1:5000)

Anti-Pyruvate Kinase Antibody has been assayed against 1.0 ug of Pyruvate Kinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:3000 to 1:15000 of the reconstitution concentration is suggested for Anti-Pyruvate Kinase Antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Reconstitute in 100 µL of deionized water.

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.