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Antibodies > PKM / Pyruvate Kinase, Muscle > LS-C98973
 
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Catalog Number Size Price
LS-C98973-200 200 µl $393 
PKM / Pyruvate Kinase, Muscle Antibody - Pyruvate Kinase (PKM2) Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human hepatocarcinoma followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of the Pyruvate Kinase (PKM2) Antibody for immunohistochemistry.
PKM / Pyruvate Kinase, Muscle Antibody - Fluorescent confocal image of MCF7 cells stained with Pyruvate Kinase (PKM2) antibody. MCF7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated Pyruvate Kinase (PKM2) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the Pyruvate Kinase (PKM2) mainly to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000067225).
PKM / Pyruvate Kinase, Muscle Antibody - Western blot of anti-h PKM2-N491 antibody in Ramos cell line lysates (35 ug/lane). hPKM2-N491(arrow) was detected using the purified antibody.
PKM / Pyruvate Kinase, Muscle Antibody - Pyruvate Kinase (PKM2) Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human hepatocarcinoma followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of the Pyruvate Kinase (PKM2) Antibody for immunohistochemistry.
PKM / Pyruvate Kinase, Muscle Antibody - Fluorescent confocal image of MCF7 cells stained with Pyruvate Kinase (PKM2) antibody. MCF7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated Pyruvate Kinase (PKM2) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the Pyruvate Kinase (PKM2) mainly to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000067225).
PKM / Pyruvate Kinase, Muscle Antibody - Western blot of anti-h PKM2-N491 antibody in Ramos cell line lysates (35 ug/lane). hPKM2-N491(arrow) was detected using the purified antibody.
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Polyclonal Rabbit anti‑Human PKM / Pyruvate Kinase, Muscle Antibody (aa476‑505, IHC, IF, WB) LS‑C98973

Polyclonal Rabbit anti‑Human PKM / Pyruvate Kinase, Muscle Antibody (aa476‑505, IHC, IF, WB) LS‑C98973

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Antibody:
PKM / Pyruvate Kinase, Muscle Rabbit anti-Human Polyclonal (aa476-505) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse, Rat, Primate
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C98973-200
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PKM / Pyruvate Kinase, Muscle Rabbit anti-Human Polyclonal (aa476-505) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse, Rat, Primate
Format:
Unconjugated, Unmodified

Specifications

Description
Pyruvate Kinase, Muscle antibody LS-C98973 is an unconjugated rabbit polyclonal antibody to Pyruvate Kinase, Muscle (PKM) (aa476-505) from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC and WB.
Target
Human PKM / Pyruvate Kinase, Muscle
Synonyms
PKM | p58 | PK3 | PKM2 | Pyruvate Kinase | Pyruvate kinase 2/3 | Pyruvate kinase, m1 | Pyruvate kinase, muscle | TCB | Tumor M2-PK | OIP-3 | OIP3 | Opa-interacting protein 3 | CTHBP | PK, muscle type | PK2 | Pyruvate kinase isozymes M1/M2 | Pyruvate kinase muscle isozyme | THBP1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Primate (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa476-505
Specificity
This Pyruvate Kinase (PKM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-505 amino acids from the C-terminal region of human Pyruvate Kinase (PKM2).
Applications
  • IHC
  • IHC - Paraffin
  • Immunofluorescence (1:200)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PKM / Pyruvate Kinase, Muscle
P14618 NM_002654 NP_002645.3

Publications (0)

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Featured Products

PKM / Pyruvate Kinase, Muscle Antibody - Anti-PKM antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ PKM / Pyruvate Kinase, Muscle Antibody LS‑B1450
Species: Rabbit, Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
PKM / Pyruvate Kinase, Muscle Antibody - Anti-PKM2 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
IHC‑plus™ PKM / Pyruvate Kinase, Muscle Antibody LS‑B2445
Species: Human, Monkey, Mouse, Rat
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot
PKM / Pyruvate Kinase, Muscle Antibody - The anti-PKM2 antibody is used in Western blot to detect PKM2 in HeLa cell lysate (Lane 1) and 3T3 cell lysate (Lane 2).
PKM / Pyruvate Kinase, Muscle Antibody (aa121‑151) LS‑C98972
Species: Human, Mouse
Applications: Western blot
PKM / Pyruvate Kinase, Muscle Antibody - Immunohistochemistry of paraffin-embedded human kidney using PKM / Pyruvate Kinase antibody at dilution of 1:100. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PKM / Pyruvate Kinase, Muscle Antibody (Biotin) LS‑C317945
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
PKM / Pyruvate Kinase, Muscle Antibody - PKM2 / Pyruvate Kinase antibody Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PKM / Pyruvate Kinase, Muscle Antibody (aa391‑440) LS‑C117151
Species: Human
Applications: Western blot
PKM / Pyruvate Kinase, Muscle ELISA Kit
Human PKM / Pyruvate Kinase, Muscle (Sandwich ELISA) ELISA Kit - LS-F4122
Reactivity: Human
Range: 78.13-5000 pg/ml
See all PKM / Pyruvate Kinase, Muscle Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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