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Anti-PKM / Pyruvate Kinase, Muscle Antibody (aa434-531) LS-C150140

Ordering

Wt. Vol. Conc. Price
100 µg - - Unavailable

Most Popular PKM / Pyruvate Kinase, Muscle Antibodies

Anti-PKM / Pyruvate Kinase, Muscle Antibody IHC-plus™ LS-B1450
Goat Polyclonal to Rabbit PKM / Pyruvate Kinase, Muscle
Rabbit, Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-PKM / Pyruvate Kinase, Muscle Antibody IHC-plus™ LS-B2445
Rabbit Polyclonal (IgG) to Human PKM / Pyruvate Kinase, Muscle
Human, Monkey, Mouse, Rat
IHC - Paraffin, IHC - Frozen, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PKM / Pyruvate Kinase, Muscle Antibody (aa121-151) LS-C98972
Rabbit Polyclonal to Human PKM / Pyruvate Kinase, Muscle
Human, Mouse
Western blot
Unconjugated
Western blot Image
Anti-PKM / Pyruvate Kinase, Muscle Antibody (aa476-505) LS-C98973
Rabbit Polyclonal to Human PKM / Pyruvate Kinase, Muscle
Human, Mouse, Rat, Primate
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PKM / Pyruvate Kinase, Muscle Antibody (aa391-440) LS-C117151
Rabbit Polyclonal to Human PKM / Pyruvate Kinase, Muscle
Human, Mouse, Rat, Guinea pig, Xenopus
Western blot
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Sheep Polyclonal (IgG) to Human PKM / Pyruvate Kinase, Muscle
Human, Mouse, Rat
ICC, Western blot
Unconjugated

Details

Human PKM / Pyruvate Kinase, Muscle
Sheep
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • ICC (5 - 15 µg/ml)
  • Western blot (0.2 µg/ml)

Specificity and Use

PKM / Pyruvate Kinase, Muscle antibody was raised against e. coli-derived recombinant human PKM2 Ser434-Pro531 Accession # P14618.
aa434-531
Detects human, mouse, rat PKM2 in Western blots and detects recombinant human PKM2 in direct ELISAs.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About PKM / Pyruvate Kinase, Muscle

P14618 NM_002654 NP_002645.3

PKM Antibody, p58 Antibody, PK3 Antibody, PKM2 Antibody, Pyruvate Kinase Antibody, Pyruvate kinase 2/3 Antibody, Pyruvate kinase, m1 Antibody, Pyruvate kinase, muscle Antibody, TCB Antibody, Tumor M2-PK Antibody, OIP-3 Antibody, OIP3 Antibody, Opa-interacting protein 3 Antibody, CTHBP Antibody, PK, muscle type Antibody, PK2 Antibody, Pyruvate kinase isozymes M1/M2 Antibody, Pyruvate kinase muscle isozyme Antibody, THBP1 Antibody

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones.

Immunocytochemistry

Immunocytochemistry
PKM2 in Wi-38 Human Cell Line. PKM2 was detected in immersion fixed Wi-38 human lung fibroblast cell line using Sheep Anti-Human/Mouse/Rat PKM2 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Immunocytochemistry

Immunocytochemistry
PKM2 in RAW 264.7 Mouse Cell Line. PKM2 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Sheep Anti-Human/Mouse/Rat PKM2 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml for 3 hours at room temperature. Cells were stained using NorthernLights 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Western blot

Western blot
Detection of Human, Mouse, and Rat PKM2 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line, KG-1 human acute myelogenous leukemia cell line, BaF3 mouse pro-B cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 ug/ml of Sheep Anti-Human/Mouse/Rat PKM2 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for PKM2 at approximately 60 kD (as indicated). This experiment was conducted under reducing conditions.

Requested From: 
Date Requested: 4/25/2017

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