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PKM / Pyruvate Kinase, Muscle Antibody (aa391‑440) LS‑C117151
Pyruvate Kinase, Muscle antibody LS-C117151 is an unconjugated rabbit polyclonal antibody to Pyruvate Kinase, Muscle (PKM) from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C117151-50
50 µg
Unavailable

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Product Description

Pyruvate Kinase, Muscle antibody LS-C117151 is an unconjugated rabbit polyclonal antibody to Pyruvate Kinase, Muscle (PKM) from human, mouse, rat and other species. Validated for WB.
About PKM / Pyruvate Kinase, Muscle
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. P14618 NM_002654 NP_002645.3

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Specifications

Target
Human PKM / Pyruvate Kinase, Muscle
Host
Rabbit
Reactivity
Human, Mouse, Rat, Guinea pig, Xenopus (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bovine, Horse, Pig, Rabbit, Chicken, Zebrafish, Drosophila (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa391-440 of human PKM (P11980, NP_002645). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Mouse, Rat, Guinea pig, Xenopus (100%); Marmoset, Elephant, Panda, Cat, Bovine, Rabbit, Horse, Pig, Turkey, Chicken, Salmon, Pufferfish, Zebrafish, Drosophila, Beetle (92%); Platypus (85%).
Blocking Peptide
LS-E21118 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C117151. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa391-440
Specificity
Human PKM2
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

PKM2 / Pyruvate Kinase antibody LS-C117151 Western blot of Fetal Heart lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PKM2 / Pyruvate Kinase antibody LS-C117151 Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PKM2 / Pyruvate Kinase antibody LS-C117151 Western blot of Fetal Heart lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PKM2 / Pyruvate Kinase antibody LS-C117151 Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PKM2 / Pyruvate Kinase antibody LS-C117151 Western blot of Fetal Heart lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PKM2 / Pyruvate Kinase antibody LS-C117151 Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

PKM2 / Pyruvate Kinase antibody LS-C117151 Western blot of Fetal Heart lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
PKM2 / Pyruvate Kinase antibody LS-C117151 Western blot of Fetal Heart lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/24/2018

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