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Anti-PKLR Antibody (clone 1F3) LS-C115401

Catalog Size Price
LS-C115401-100 100 µl Unavailable

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Immunofluorescence Image
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100% Guaranteed
Mouse Monoclonal [clone 1F3] (IgG2a) to Human PKLR
Human
Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human PKLR
Mouse
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [1F3]
Unconjugated
Protein A/G purified
Unmodified

Applications

  • Immunofluorescence (1:100)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)

Specificity and Use

PKLR antibody was raised against full length human recombinant protein of human PKLR (NP_000289) produced in HEK293T cell.
Human PKLR
Concentration: 0.5-1.0 mg/ml (Lot dependent)

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About PKLR

P30613 NM_000298 NP_000289.1

PKLR Antibody, PKL Antibody, Pyruvate kinase, liver and RBC Antibody, Pyruvate kinase type L Antibody, Red cell/liver pyruvate kinase Antibody, RPK Antibody, PK1 Antibody, PKR Antibody, Pyruvate kinase 1 Antibody, Pyruvate kinase isozymes R/L Antibody, R-type/L-type pyruvate kinase Antibody, PKRL Antibody, Pyruvate kinase isozyme R/L Antibody

PKLR is a pyruvate kinase that catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP, which is the rate-limiting step of glycolysis. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Multiple transcript variants encoding different isoforms have been found for this gene.

Immunofluorescence

Immunofluorescence
Anti-PKLR mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PKLR.

Western blot

Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKLR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKLR.

Flow Cytometry

Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PKLR antibody, and then analyzed by flow cytometry.

Requested From: 
Date Requested: 12/11/2017

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