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Anti-PKG / PRKG1 Antibody (aa657-671) IHC-plus™ LS-B2219

Note: This antibody replaces LS-C38517

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular PKG / PRKG1 Antibodies

Anti-PKG / PRKG1 Antibody (aa657-671) IHC-plus™ LS-B2219
Rabbit Polyclonal (IgG) to Human PKG / PRKG1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-PKG / PRKG1 Antibody (clone 2B3) IHC-plus™ LS-B4398
Mouse Monoclonal [clone 2B3] (IgG1,k) to Human PKG / PRKG1
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-PKG / PRKG1 Antibody LS-C343645
Rabbit Polyclonal to Human PKG / PRKG1
Human, Mouse, Rat, Bovine
IHC, ICC, Western blot, Immunoprecipitation
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal (IgG) to Human PKG / PRKG1
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken
IHC - Paraffin, Western blot
Unconjugated

Details

Human PKG / PRKG1
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 µg/ml)

Specificity and Use

PKG / PRKG1 antibody was raised against a 15 residue synthetic peptide DEPPPDDNSGWDIDF based on the human PKG 1alpha (residues 657-671) and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%); Pufferfish (93%); Xenopus, Zebrafish (87%); Stickleback, Medaka, Seq squirt, Mosquito, Ant, Beetle (80%).
aa657-671
Recognizes human PKG1 alpha at ~75kD.
Immunohistochemistry: LS-B2219 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2219 was determined to be 10 ug/ml. Western Blot: Positive control: Mouse Brain Tissue Extract, Rat Brain Tissue Extract.

Packaging

PBS, pH 7.2, 50% glycerol, 0.09% azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

Publications (1)

1
Cyclic guanosine monophosphate and the dependent protein kinase regulate lymphatic contractility in rat thoracic duct. Gasheva OY, Gashev AA, Zawieja DC. The Journal of physiology. 2013 Sep;591:4549-65. [Full Text Article] [PubMed:23836689] [PMC:PMC3784198]

About PKG / PRKG1

Q13976 NM_006258 NP_006249.1

PRKG1 Antibody, CGKI Antibody, CGMP kinase I Antibody, CGMP protein kinase type I Antibody, CGK-I Antibody, CGK1 Antibody, PKGI Antibody, PKG I Antibody, PRKGR1B Antibody, PRKG1B Antibody, PRKGR1A Antibody, PGK Antibody, PKG Antibody, CGK Antibody, CGK 1 Antibody, CGKI-alpha Antibody, CGKI-BETA Antibody

Serine/threonine protein kinase that acts as key mediator of the nitric oxide (NO)/cGMP signaling pathway. GMP binding activates PRKG1, which phosphorylates serines and threonines on many cellular proteins. Numerous protein targets for PRKG1 phosphorylation are implicated in modulating cellular calcium, but the contribution of each of these targets may vary substantially among cell types.

Immunohistochemistry

Immunohistochemistry
Anti-PRKG1 / CGKI antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2219 concentration 10 ug/ml.
 

Requested From: United States
Date Requested: 12/10/2016

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